| Product name | Rabbit anti-Sheep TNF-alpha mAb(CAP) |
|---|---|
| Catalog No. | RM18031 |
| Catagory | Elisa Antibody Kit |
| Application | ELISA |
| Description | Cytokine that binds to TNFRSF1A/TNFR1 and TNFRSF1B/TNFBR. It is mainly secreted by macrophages and can induce cell death of certain tumor cell lines. It is potent pyrogen causing fever by direct action or by stimulation of interleukin-1 secretion and is implicated in the induction of cachexia, Under certain conditions it can stimulate cell proliferation and induce cell differentiation (By similarity).Induces insulin resistance in adipocytes via inhibition of insulin-induced IRS1 tyrosine phosphorylation and insulin-induced glucose uptake. Induces GKAP42 protein degradation in adipocytes which is partially responsible for TNF-induced insulin resistance (By similarity).Plays a role in angiogenesis by inducing VEGF production synergistically with IL1B and IL6 (By similarity).The TNF intracellular domain (ICD) form induces IL12 production in dendritic cells. |
| Immunogen | Recombinant Sheep TNF-alpha |
| Purirty | Affinity purification |
| Ig Type | Rabbit IgG |
| Formulation | Supplied as a 0.2umfiltered solution in PBS with0.05%ProClin 300 , Ph7.4 containing sheep TNF-alpha Antibody. |
| CrossReactivity | / |
| Storage | Store at -20℃. Avoid freeze / thaw cycles. Preservative：0.05%ProClin 300. Avoid repeated freeze-thaw cycles. |
Sheep TNF-alpha Sandwich ELISA Immunoassay
| Recommended Concentration | Sample | |
|---|---|---|
| ELISA Capture | 1-4ug/mL | Rabbit anti-Sheep TNF-alpha (CAP)(Cat. No.RM18031) |
| ELISA Detection | 0.02-0.08ug/mL | Rabbit anti-Sheep TNF-alpha (DET)(Cat. No.RM18032) |
| Standard | 15.63-1000pg/mL | Recombinant Sheep TNF-alpha Protein |
