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Biotinylated Recombinant Human CD8 alpha Protein (RP00587B)

Datasheet

Overview

Product nameBiotinylated Recombinant Human CD8 alpha Protein
Catalog No.RP00587B
DescriptionBiotinylated Recombinant Human CD8 alpha Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Ser22-Asp182) of Human CD8 alpha (Accession #P01732-1) fused with a C-His&Avi tag at the C-terminus.
Purity> 95% by SDS-PAGE
Endotoxin< 1 EU/μg of the protein by LAL method
FormulationLyophilized from 0.22 μm filtered solution in PBS (pH 7.4). Normally 8% trehalose is added as protectant before lyophilization.
SpeciesHuman
Calculated MW20.5 kDa
CD8 alpha enhances the responses of antigen-specific CTL activated through TCR through binding MHC class I, favoring lipid raft partitioning of TCR, and inducing intracellular signaling. CD8 alpha is also found on dendritic cells and rat macrophages, but whether CD8 alpha enhances responses of a partner receptor, like TCR, to activate these cells is not known.
SynonymCD8a; Lyt-2; CD8; Leu2; MAL; p32; CD8a molecule; OKT8 T-cell antigen
Expression HostHEK293 cells
TagC-His&Avi
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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