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CD8A Rabbit mAb (A22219)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunohistochemistry - CD8A Rabbit mAb (A22219)

Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using CD8A Rabbit mAb (A22219) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - CD8A Rabbit mAb (A22219)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD8A Rabbit mAb (A22219) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

Overview

Product nameCD8A Rabbit mAb
Catalog No.A22219
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55249
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
SequenceSQFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACD
Gene ID
Swiss Prot
SynonymsCD8; p32; Leu2; CD8alpha; CD8A
Calculated MW21kDa/25kDa/30kDa
Observed MWRefer to figures
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunohistochemistry    
Positive samples
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.

Documents

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ABclonal:Immunohistochemistry - CD8A Rabbit mAb (A22219)}

Immunohistochemistry - CD8A Rabbit mAb (A22219)

Immunohistochemistry analysis of paraffin-embedded Human appendix tissue using CD8A Rabbit mAb (A22219) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - CD8A Rabbit mAb (A22219)}

Immunohistochemistry - CD8A Rabbit mAb (A22219)

Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using CD8A Rabbit mAb (A22219) at a dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Buffer (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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