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ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220)

Flow cytometry: 1X10^6 293F cells (negative control, left) and Human PBMC (right) were surface-stained with ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220, 5 μl/Test, right).

Overview

Product nameABflo® 450 Rabbit anti-Human/Monkey CD8a mAb
Catalog No.A27220
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC55248-ABflo450
The CD8 antigen is a cell surface glycoprotein found on most cytotoxic T lymphocytes that mediates efficient cell-cell interactions within the immune system. The CD8 antigen acts as a coreceptor with the T-cell receptor on the T lymphocyte to recognize antigens displayed by an antigen presenting cell in the context of class I MHC molecules. The coreceptor functions as either a homodimer composed of two alpha chains or as a heterodimer composed of one alpha and one beta chain. Both alpha and beta chains share significant homology to immunoglobulin variable light chains. This gene encodes the CD8 alpha chain. Multiple transcript variants encoding different isoforms have been found for this gene. The major protein isoforms of this gene differ by the presence or absence of a transmembrane domain and thus differ in being a membrane-anchored or secreted protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-182 of human CD8A (NP_001759.3).
SequenceSQFRVSPLDRTWNLGETVELKCQVLLSNPTSGCSWLFQPRGAAASPTFLLYLSQNKPKAAEGLDTQRFSGKRLGDTFVLTLSDFRRENEGYYFCSALSNSIMYFSHFVPVFLPAKPTTTPAPRPPTPAPTIASQPLSLRPEACRPAAGGAVHTRGLDFACD
Gene ID
Swiss Prot
SynonymsCD8; p32; Leu2; CD8alpha
Calculated MW21kDa/25kDa/30kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Secreted, Single-pass type I membrane protein.

Documents

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ABclonal:Flow CytoMetry - ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220)}

Flow CytoMetry - ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220)

Flow cytometry: 1X10^6 293F cells (negative control, left) and Human PBMC (right) were surface-stained with ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220)}

Flow CytoMetry - ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (5 μl/Test, left) or ABflo® 450 Rabbit anti-Human/Monkey CD8a mAb (A27220, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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