Recombinant Human CD14 Protein (RP00990)

Product name	Recombinant Human CD14 Protein
Catalog No.	RP00990
Description	Recombinant Human CD14 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Thr20-Cys352) of human CD14 (Accession #NP_000582.1) fused with a 6×His tag at the C-terminus.
Bio-Activity	Measured by its ability to enhance LPS-stimulated IL-8 secretion by THP-1 human acute monocytic leukemia cells. The ED₅₀ for this effect is 5.7-23 ng/mL.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Human
Calculated MW	36.61 kDa

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.