제품 > 항체 > 단일클론항체(mAb)

ABflo® 450 Rabbit anti-Human CD14 mAb (A25196)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Flow cytometry: 1X10^6 HUVEC cells (negative control, left) and Human PBMC (right) were surface-stained with ABflo® 450 Rabbit anti-Human CD14 mAb (A25196, 5 μl/Test, orange line) or ABflo® 450 Rabbit IgG isotype control (A24171, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (A24171, 5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD14 mAb (A25196, 5 μl/Test, right).

Overview

Product name	ABflo® 450 Rabbit anti-Human CD14 mAb
Catalog No.	A25196
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC65657-ABf450

Background

The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 20-352 of human CD14 (NP_000582.1).
Sequence	TTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMNSGVVPAC
Gene ID
Swiss Prot
Synonyms	CD14
Calculated MW	40kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Cell membrane, GPI-anchor, Lipid-anchor, Secreted

Documents

Certificate of Compliance

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Research Area

ABflo® 450 Rabbit anti-Human CD14 mAb images

Flow CytoMetry - ABflo® 450 Rabbit anti-Human CD14 mAb (A25196)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 450 Rabbit IgG isotype control (A24171, 5 μl/Test, left) or ABflo® 450 Rabbit anti-Human CD14 mAb (A25196, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.