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ABflo® 647 Rabbit anti-Human CD14 mAb (A25072)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD14 mAb (A25072)

Flow cytometry: 1X10^6 HUVEC cells (negative control, left) and Human PBMC (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD14 mAb (A25072, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD14 mAb (A25072)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD14 mAb (A25072, 5 μl/Test, right).

Overview

Product nameABflo® 647 Rabbit anti-Human CD14 mAb
Catalog No.A25072
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC65657-ABflo647
The protein encoded by this gene is a surface antigen that is preferentially expressed on monocytes/macrophages. It cooperates with other proteins to mediate the innate immune response to bacterial lipopolysaccharide, and to viruses. This gene has been identified as a target candidate in the treatment of SARS-CoV-2-infected patients to potentially lessen or inhibit a severe inflammatory response. Alternative splicing results in multiple transcript variants encoding the same protein.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 20-352 of human CD14 (NP_000582.1).
SequenceTTPEPCELDDEDFRCVCNFSEPQPDWSEAFQCVSAVEVEIHAGGLNLEPFLKRVDADADPRQYADTVKALRVRRLTVGAAQVPAQLLVGALRVLAYSRLKELTLEDLKITGTMPPLPLEATGLALSSLRLRNVSWATGRSWLAELQQWLKPGLKVLSIAQAHSPAFSCEQVRAFPALTSLDLSDNPGLGERGLMAALCPHKFPAIQNLALRNTGMETPTGVCAALAAAGVQPHSLDLSHNSLRATVNPSAPRCMWSSALNSLNLSFAGLEQVPKGLPAKLRVLDLSCNRLNRAPQPDELPEVDNLTLDGNPFLVPGTALPHEGSMNSGVVPAC
Gene ID
Swiss Prot
SynonymsCD14
Calculated MW40kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, GPI-anchor, Lipid-anchor, Secreted

Documents

Certificate of Compliance

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ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD14 mAb (A25072)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD14 mAb (A25072)

Flow cytometry: 1X10^6 HUVEC cells (negative control, left) and Human PBMC (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD14 mAb (A25072, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD14 mAb (A25072)}

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD14 mAb (A25072)

Flow cytometry: 1X10^6 Human PBMC were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD14 mAb (A25072, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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Secondary Antibodies (26)