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Recombinant Human PCSK9 Protein (RP01148)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Human PCSK9 Protein
Catalog No.RP01148
DescriptionRecombinant Human PCSK9 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln31-Gln692) of human PCSK9 (Accession #NP_777596.2) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human LDLR at 5μg/mL (100 μL/well) can bind Human PCSK9 with a linear range of 0.5-294.7ng/mL.
Purity> 92% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW71.89 kDa
SynonymFH3; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9; PCSK9; FH3; proprotein convertase subtilisin/kexin type 9; HCHOLA3; LDLCQ1; NARC-1; NARC1; PC9
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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