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Recombinant Human Semaphorin-4D/SEMA4D/CD100 Protein (RP00981)

Bioactivityvalidated

Publication (1) Datasheet

Overview

Product nameRecombinant Human Semaphorin-4D/SEMA4D/CD100 Protein
Catalog No.RP00981
DescriptionRecombinant Human Semaphorin-4D/SEMA4D/CD100 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Met22-Arg734) of human Semaphorin 4D (Accession #NP_006369.3) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by its binding ability in a functional ELISA. Immobilized Human SEMA4D Protein at 1μg/mL (100 μL/well) can bind CD100 Rabbit mAb with a linear range of 0.012-0.88 ng/mL.
Purity> 97% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesHuman
Calculated MW80.00 kDa
This protein is a member of the semaphorin family of proteins and an important mediator of the movement and differentiation of multiple cell types, including those of the immune, vascular, and nervous systems. VEGF and SEMA4D had a positive correlation with the malignant degree of ovarian cancer, and SEMA4D can serve as an independent prognostic factor. SEMA4D was the first semaphorin described to have immune functions and serves important roles in T cell priming, antibody production, and cell-to-cell adhesion. Proteolytic cleavage of SEMA4D from the cell surface gives rise to a soluble fragment of SEMA4D (sSEMA4D). Similar to the transmembranal form, sSEMA4D is thought to have immunoregulatory properties.
SynonymSEMA4D; C9orf164; CD100; M-sema-G; SEMAJ; coll-4; Semaphorin 4D / CD100
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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