제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb (A24406)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human

Confocal imaging of MOLT-4 cells using ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb (A24406, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb (A24406)

Flow cytometry:1X10^6 PC-3 cells (Low Expression, left) and MOLT-4 cells (right) were surface-stained with ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb(A24406, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry:1X10^6 MOLT-4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb(A24406, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb
Catalog No.	A24406
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63157-ABflo647

Background

Plexin-B1 is related to axon guidance in the central nervous system. The Sema4D function is also widely detected in the immune system and was found to be the first semaphorin expressed on the surface of many types of immune cells. In the immune system, CD72 is a low-affinity receptor for Sema4D, and studies have shown that Sema4D can not only regulate T cell activation, but also participate in the regulation of B cell survival and differentiation. In the immune system, soluble fragments containing extracellular domains produced by proteolytic cleavage can regulate many physiological functions of Sema4D. Sema4D is also associated with tumorigenesis because studies have confirmed that it is overexpressed in various types of solid tumor cells. To some extent, the role of Sema4D in tumorigenesis is related to its ability to cause tumor angiogenesis, cell invasion, and immunosuppression by enhancing bone marrow-derived suppressor cell function.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 22-734 of human Semaphorin-4D/CD100(NP_006369.3).
Sequence	MAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNR
Gene ID
Swiss Prot
Synonyms	SEMA4D; C9orf164; CD100; M-sema-G; SEMAJ; coll-4; semaphorin-4D
Calculated MW	82kDa/96kDa
Observed MW

Applications

Reactivity	Human
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	IF/ICC 1:50 - 1:200 FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Immunofluorescence Flow Cytometry
Positive samples
Cellular location	Cell membrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Research Area

ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb images

Immunofluorescence - ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb (A24406)

Confocal imaging of MOLT-4 cells using ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb (A24406, dilution 1:50). DAPI was used for nuclear staining (Blue). Objective: 100x.

Flow CytoMetry - ABflo® 647 Rabbit anti-Human CD100/SEMA4D mAb (A24406)

* For research use only. Not for therapeutic or diagnostic purposes.