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Semaphorin-4D/CD100 Rabbit mAb (A24404)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Immunofluorescence - Semaphorin-4D/CD100 Rabbit mAb (A24404)

Confocal imaging of MOLT-4 cells using Semaphorin-4D/CD100 Rabbit mAb (A24404, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - Semaphorin-4D/CD100 Rabbit mAb (A24404)

Flow cytometry: 1X10^6 PC-3 cells (Low Expression, left) and MOLT-4 cells (right) were surface-stained with Semaphorin-4D/CD100 Rabbit mAb(A24404, 2μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2μg/mL, blue line), followed by Alexa Fluor®647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - Semaphorin-4D/CD100 Rabbit mAb (A24404)

Flow cytometry:1X10^6 MOLT-4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or Semaphorin-4D/CD100 Rabbit mAb(A24404, 2 μg/mL, right).

Overview

Product nameSemaphorin-4D/CD100 Rabbit mAb
Catalog No.A24404
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63157
Plexin-B1 is related to axon guidance in the central nervous system. The Sema4D function is also widely detected in the immune system and was found to be the first semaphorin expressed on the surface of many types of immune cells. In the immune system, CD72 is a low-affinity receptor for Sema4D, and studies have shown that Sema4D can not only regulate T cell activation, but also participate in the regulation of B cell survival and differentiation. In the immune system, soluble fragments containing extracellular domains produced by proteolytic cleavage can regulate many physiological functions of Sema4D. Sema4D is also associated with tumorigenesis because studies have confirmed that it is overexpressed in various types of solid tumor cells. To some extent, the role of Sema4D in tumorigenesis is related to its ability to cause tumor angiogenesis, cell invasion, and immunosuppression by enhancing bone marrow-derived suppressor cell function.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-734 of human Semaphorin-4D/CD100(NP_006369.3).
SequenceMAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNR
Gene ID
Swiss Prot
SynonymsSEMA4D; C9orf164; CD100; M-sema-G; SEMAJ; coll-4; semaphorin-4D; Semaphorin-4D/CD100
Calculated MW82kDa/96kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50-1:200
  • FC 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein.

Documents

Certificate of Compliance

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Lot number

ABclonal:Immunofluorescence - Semaphorin-4D/CD100 Rabbit mAb (A24404)}

Immunofluorescence - Semaphorin-4D/CD100 Rabbit mAb (A24404)

Confocal imaging of MOLT-4 cells using Semaphorin-4D/CD100 Rabbit mAb (A24404, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 100x.
ABclonal:Flow CytoMetry - Semaphorin-4D/CD100 Rabbit mAb (A24404)}

Flow CytoMetry - Semaphorin-4D/CD100 Rabbit mAb (A24404)

Flow cytometry: 1X10^6 PC-3 cells (Low Expression, left) and MOLT-4 cells (right) were surface-stained with Semaphorin-4D/CD100 Rabbit mAb(A24404, 2μg/mL, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 2μg/mL, blue line), followed by Alexa Fluor®647 conjugated goat anti-rabbit pAb staining. Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - Semaphorin-4D/CD100 Rabbit mAb (A24404)}

Flow CytoMetry - Semaphorin-4D/CD100 Rabbit mAb (A24404)

Flow cytometry:1X10^6 MOLT-4 cells were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 2 μg/mL, left) or Semaphorin-4D/CD100 Rabbit mAb(A24404, 2 μg/mL, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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