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PE Rabbit anti-Human CD100/SEMA4D mAb (A26506)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human

ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD100/SEMA4D mAb (A26506)

Flow cytometry: 1X10^6 PC-3 cells (Low Expression, left) and MOLT-4 cells (right) were surface-stained with PE Rabbit anti-Human CD100/SEMA4D mAb (A26506, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD100/SEMA4D mAb (A26506)

Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD100/SEMA4D mAb (A26506, 5 μl/Test, right).

Overview

Product namePE Rabbit anti-Human CD100/SEMA4D mAb
Catalog No.A26506
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
CloneNo.ARC63157-PE
Plexin-B1 is related to axon guidance in the central nervous system. The Sema4D function is also widely detected in the immune system and was found to be the first semaphorin expressed on the surface of many types of immune cells. In the immune system, CD72 is a low-affinity receptor for Sema4D, and studies have shown that Sema4D can not only regulate T cell activation, but also participate in the regulation of B cell survival and differentiation. In the immune system, soluble fragments containing extracellular domains produced by proteolytic cleavage can regulate many physiological functions of Sema4D. Sema4D is also associated with tumorigenesis because studies have confirmed that it is overexpressed in various types of solid tumor cells. To some extent, the role of Sema4D in tumorigenesis is related to its ability to cause tumor angiogenesis, cell invasion, and immunosuppression by enhancing bone marrow-derived suppressor cell function.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 22-734 of human Semaphorin-4D/CD100(NP_006369.3).
SequenceMAFAPIPRITWEHREVHLVQFHEPDIYNYSALLLSEDKDTLYIGAREAVFAVNALNISEKQHEVYWKVSEDKKAKCAEKGKSKQTECLNYIRVLQPLSATSLYVCGTNAFQPACDHLNLTSFKFLGKNEDGKGRCPFDPAHSYTSVMVDGELYSGTSYNFLGSEPIISRNSSHSPLRTEYAIPWLNEPSFVFADVIRKSPDSPDGEDDRVYFFFTEVSVEYEFVFRVLIPRIARVCKGDQGGLRTLQKKWTSFLKARLICSRPDSGLVFNVLRDVFVLRSPGLKVPVFYALFTPQLNNVGLSAVCAYNLSTAEEVFSHGKYMQSTTVEQSHTKWVRYNGPVPKPRPGACIDSEARAANYTSSLNLPDKTLQFVKDHPLMDDSVTPIDNRPRLIKKDVNYTQIVVDRTQALDGTVYDVMFVSTDRGALHKAISLEHAVHIIEETQLFQDFEPVQTLLLSSKKGNRFVYAGSNSGVVQAPLAFCGKHGTCEDCVLARDPYCAWSPPTATCVALHQTESPSRGLIQEMSGDASVCPDKSKGSYRQHFFKHGGTAELKCSQKSNLARVFWKFQNGVLKAESPKYGLMGRKNLLIFNLSEGDSGVYQCLSEERVKNKTVFQVVAKHVLEVKVVPKPVVAPTLSVVQTEGSRIATKVLVASTQGSSPPTPAVQATSSGAITLPPKPAPTGTSCEPKIVINTVPQLHSEKTMYLKSSDNR
Gene ID
Swiss Prot
SynonymsA8; GR3; BB18; CD100; COLL4; SEMAJ; coll-4; C9orf164; M-sema-G
Calculated MW82kDa/96kDa
Observed MW
ReactivityHuman
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 5 μl per 10^6 cells in 100 μl volume
Storage bufferStore at 2-8℃. Avoid freeze.
Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular locationCell membrane, Single-pass type I membrane protein.

Documents

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ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD100/SEMA4D mAb (A26506)}

Flow CytoMetry - PE Rabbit anti-Human CD100/SEMA4D mAb (A26506)

Flow cytometry: 1X10^6 PC-3 cells (Low Expression, left) and MOLT-4 cells (right) were surface-stained with PE Rabbit anti-Human CD100/SEMA4D mAb (A26506, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).
ABclonal:Flow CytoMetry - PE Rabbit anti-Human CD100/SEMA4D mAb (A26506)}

Flow CytoMetry - PE Rabbit anti-Human CD100/SEMA4D mAb (A26506)

Flow cytometry: 1X10^6 MOLT-4 cells were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Human CD100/SEMA4D mAb (A26506, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.

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항체 (5)

Secondary Antibodies (26)