Recombinant Mouse ICAM-1/CD54 Protein (RP01056)

Product name	Recombinant Mouse ICAM-1/CD54 Protein
Catalog No.	RP01056
Description	Recombinant Mouse ICAM-1/CD54 Protein is produced by HEK293 expression system. The target protein is expressed with sequence (Gln28-Asn485) of mouse ICAM-1/CD54 (Accession #NP_034623.1. ) fused with an Fc, 6×His tag at the C-terminus.
Bio-Activity	Measured by the ability of the immobilized protein to support the adhesion of THP-1 cells. When cells are added to ICAM-1 coated plates (12.5 μg/mL, 100 μL/well), approximately >80% cells will adhere specifically after 60 minutes at 37 ℃.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 0.1 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
Species	Mouse
Calculated MW	77.03 kDa

Synonym	ICAM1; BB2; CD54; P3.58; MAL; CD54; Icam; Ly-4; Ly-47; Icam-1; MALA-2
Expression Host	HEK293 cells
Tag	C-hFc&His
Swiss-Prot
Gene ID

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.