제품 > 항체 > 단일클론항체(mAb)

PE Rabbit anti-Mouse/Rat CD54 mAb (A25962)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Flow cytometry: 1X10^6 C2C12 cells (negative control, left) and C57BL/6 Thymocytes (right) were surface-stained with PE Rabbit anti-Mouse/Rat CD54 mAb (A25962, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 Rat Splenocytes were surface-stained with PE Rabbit anti-Mouse/Rat CD54 mAb (A25962, 5 μl/Test, orange line) or PE Rabbit IgG isotype control (A24172, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 C57BL/6 Thymocytes were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse/Rat CD54 mAb (A25962, 5 μl/Test, right).

Flow cytometry: 1X10^6 Rat Splenocytes were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse/Rat CD54 mAb (A25962, 5 μl/Test, right).

Overview

Product name	PE Rabbit anti-Mouse/Rat CD54 mAb
Catalog No.	A25962
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63603-PE

Background

This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 28-485 of mouse CD54(NP_034623.1).
Sequence	QVSIHPREAFLPQGGSVQVNCSSSCKEDLSLGLETQWLKDELESGPNWKLFELSEIGEDSSPLCFENCGTVQSSASATITVYSFPESVELRPLPAWQQVGKDLTLRCHVDGGAPRTQLSAVLLRGEEILSRQPVGGHPKDPKEITFTVLASRGDHGANFSCRTELDLRPQGLALFSNVSEARSLRTFDLPATIPKLDTPDLLEVGTQQKLFCSLEGLFPASEARIYLELGGQMPTQESTNSSDSVSATALVEVTEEFDRTLPLRCVLELADQILETQRTLTVYNFSAPVLTLSQLEVSEGSQVTVKCEAHSGSKVVLLSGVEPRPPTPQVQFTLNASSEDHKRSFFCSAALEVAGKFLFKNQTLELHVLYGPRLDETDCLGNWTWQEGSQQTLKCQAWGNPSPKMTCRRKADGALLPIGVVKSVKQEMNGTYVCHAFSSHGNVTRNVYLTVLYHSQNN
Gene ID
Swiss Prot
Synonyms	CD54; Ly-47; Icam-1; MALA-2
Calculated MW	59kDa
Observed MW

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.09% Sodium azide, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Single-pass type I membrane protein

Documents

Certificate of Compliance

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Research Area

PE Rabbit anti-Mouse/Rat CD54 mAb images

Flow CytoMetry - PE Rabbit anti-Mouse/Rat CD54 mAb (A25962)

Flow cytometry: 1X10^6 C57BL/6 Thymocytes were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse/Rat CD54 mAb (A25962, 5 μl/Test, right).

Flow CytoMetry - PE Rabbit anti-Mouse/Rat CD54 mAb (A25962)

Flow cytometry: 1X10^6 Rat Splenocytes were surface-stained with PE Rabbit IgG isotype control (A24172, 5 μl/Test, left) or PE Rabbit anti-Mouse/Rat CD54 mAb (A25962, 5 μl/Test, right).

* For research use only. Not for therapeutic or diagnostic purposes.