제품 > 항체 > 단일클론항체(mAb)

ABflo® 647 Rabbit anti-Mouse/Rat CD54 mAb (A24895)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Flow cytometry: 1X10^6 C2C12 cells (negative control, left) and mouse thymocytes (right) were surface-stained with ABflo® 647 Rabbit anti-Mouse/Rat CD54 mAb (A24895, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained cells were used as blank control (red line).

Flow cytometry: 1X10^6 rat spleen cells were surface-stained with ABflo® 647 Rabbit anti-Mouse/Rat CD54 mAb (A24895, 5 μl/Test, orange line) or ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, blue line). Non-fluorescently stained rat spleen cells were used as blank control (red line).

Flow cytometry: 1X10^6 mouse thymocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse/Rat CD54 mAb (A24895, 5 μl/Test, right).

Flow cytometry: 1X10^6 Rat splenocytes were surface-stained with ABflo® 647 Rabbit IgG isotype control (A22070, 5 μl/Test, left) or ABflo® 647 Rabbit anti-Mouse/Rat CD54 mAb (A24895, 5 μl/Test, right).

Overview

Product name	ABflo® 647 Rabbit anti-Mouse/Rat CD54 mAb
Catalog No.	A24895
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63603-ABflo647

Background

This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 28-485 of mouse CD54(NP_034623.1).
Sequence	QVSIHPREAFLPQGGSVQVNCSSSCKEDLSLGLETQWLKDELESGPNWKLFELSEIGEDSSPLCFENCGTVQSSASATITVYSFPESVELRPLPAWQQVGKDLTLRCHVDGGAPRTQLSAVLLRGEEILSRQPVGGHPKDPKEITFTVLASRGDHGANFSCRTELDLRPQGLALFSNVSEARSLRTFDLPATIPKLDTPDLLEVGTQQKLFCSLEGLFPASEARIYLELGGQMPTQESTNSSDSVSATALVEVTEEFDRTLPLRCVLELADQILETQRTLTVYNFSAPVLTLSQLEVSEGSQVTVKCEAHSGSKVVLLSGVEPRPPTPQVQFTLNASSEDHKRSFFCSAALEVAGKFLFKNQTLELHVLYGPRLDETDCLGNWTWQEGSQQTLKCQAWGNPSPKMTCRRKADGALLPIGVVKSVKQEMNGTYVCHAFSSHGNVTRNVYLTVLYHSQNN
Gene ID
Swiss Prot
Synonyms	CD54; Ly-47; Icam-1; MALA-2
Calculated MW	59kDa
Observed MW

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	FC 5 μl per 10^6 cells in 100 μl volume
Storage buffer	Store at 2-8℃. Avoid freeze. Buffer: PBS with 0.03% proclin300, 0.2% BSA, pH7.3.
Key application	Flow Cytometry
Positive samples
Cellular location	Single-pass type I membrane protein