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ICAM-1/CD54 Rabbit mAb (A24648)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of various lysates, using ICAM-1/CD54 Rabbit mAb (A24648) at 1:6000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Negative control (NC):C2C12
Exposure time: 15s.

Western blot analysis of lysates from Rat lung, using ICAM-1/CD54 Rabbit mAb (A24648) at 1:6000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - ICAM-1/CD54 Rabbit mAb (A24648)

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using ICAM-1/CD54 Rabbit mAb (A24648) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse liver using ICAM-1/CD54 Rabbit mAb (A24648) at dilution of 1:1000 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - ICAM-1/CD54 Rabbit mAb (A24648)

Confocal imaging of rat lung using ICAM-1/CD54 Rabbit mAb (A24648, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 100x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Confocal imaging of mouse lung using ICAM-1/CD54 Rabbit mAb (A24648, at dilution of 1:200) (Red). DAPI was used for nuclear staining (blue). Objective: 100x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Overview

Product name	ICAM-1/CD54 Rabbit mAb
Catalog No.	A24648
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC63597

Background

This gene encodes an integral membrane protein that binds leukocyte adhesion protein LFA-1. It participates in the innate immune response.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 28-485 of mouse ICAM-1/CD54(NP_034623.1).
Sequence	QVSIHPREAFLPQGGSVQVNCSSSCKEDLSLGLETQWLKDELESGPNWKLFELSEIGEDSSPLCFENCGTVQSSASATITVYSFPESVELRPLPAWQQVGKDLTLRCHVDGGAPRTQLSAVLLRGEEILSRQPVGGHPKDPKEITFTVLASRGDHGANFSCRTELDLRPQGLALFSNVSEARSLRTFDLPATIPKLDTPDLLEVGTQQKLFCSLEGLFPASEARIYLELGGQMPTQESTNSSDSVSATALVEVTEEFDRTLPLRCVLELADQILETQRTLTVYNFSAPVLTLSQLEVSEGSQVTVKCEAHSGSKVVLLSGVEPRPPTPQVQFTLNASSEDHKRSFFCSAALEVAGKFLFKNQTLELHVLYGPRLDETDCLGNWTWQEGSQQTLKCQAWGNPSPKMTCRRKADGALLPIGVVKSVKQEMNGTYVCHAFSSHGNVTRNVYLTVLYHSQNN
Gene ID
Swiss Prot
Synonyms	CD54; Ly-47; Icam-1; MALA-2; ICAM-1/CD54
Calculated MW	59kDa
Observed MW	82-100kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:6000 - 1:36000 IHC-P 1:1000 - 1:4000 IF/ICC 1:200 - 1:800 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Mouse spleen, Rat lung
Cellular location	Single-pass type I membrane protein.
Customer validation	WB(Homo sapiens) qPCR(Homo sapiens,Mus musculus) WB(Homo sapiens,Mus musculus)