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Recombinant Mouse ICAM-1/CD54 Protein (RP01065)

Bioactivityvalidated

Datasheet

Overview

Product nameRecombinant Mouse ICAM-1/CD54 Protein
Catalog No.RP01065
DescriptionRecombinant Mouse ICAM-1/CD54 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Gln28-Asn485) of mouse ICAM-1/CD54 (Accession #NP_034623.1) fused with a 6×His tag at the C-terminus.
Bio-ActivityMeasured by the ability of the immobilized protein to support the adhesion of THP-1 cells. When cells are added to ICAM-1 coated plates (12.5 μg/mL, 100 μL/well), approximately >30% cells will adhere specifically after 60 minutes at 37 ℃.
Purity> 97% by SDS-PAGE.
Endotoxin< 0.1 EU/μg of the protein by LAL method.
FormulationLyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.Contact us for customized product form or formulation.
SpeciesMouse
Calculated MW51.08 kDa
SynonymICAM1; BB2; CD54; P3.58; MAL; CD54; Icam; Ly-4; Ly-47; Icam-1; MALA-2
Expression HostHEK293 cells
TagC-His
Swiss-Prot
Gene ID
StorageStore at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt.
After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
ReconstitutionCentrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

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