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Recombinant SARS-CoV-2 Nucleocapsid Protein (RP01264LQ)

Bioactivityvalidated

Publications (3) Datasheet

Overview

Product nameRecombinant SARS-CoV-2 Nucleocapsid Protein
Catalog No.RP01264LQ
DescriptionRecombinant SARS-CoV-2(2019-nCoV) Nucleocapsid Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala419) of SARS-COV-2(2019-nCoV) Nucleocapsid (Accession #QHD43423.2) fused with an 6×His tag at the N-terminus.
Bio-Activity1.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 Nucleocapsid at 2 μg/mL (100 μL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL.
2.Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2 μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL.
Purity> 95% by SDS-PAGE;> 95% by HPLC.
Endotoxin< 1.0 EU/μg of the protein by LAL method.
FormulationSupplied as a 0.22 μm filtered solution in 20mM Tris, 500mM NaCl, 0.1mM EDTA, 10% glycerol, pH8.0.Contact us for customized product form or formulation.
SpeciesSARS-CoV-2
Calculated MW46.48 kDa
SynonymNucleoprotein
Expression HostE. coli
TagN-His
Gene ID
StorageStore at -70℃. This product is stable at ≤ -70℃ for up to 1 year from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature. Avoid repeated freeze-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.

Publishing research using RP01264LQ? Please let us know so that we can cite the reference in this datasheet.

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