제품 > 항체 > 단일클론항체(mAb)

SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:SARS-CoV-2

Western blot analysis of 293T, using SARS-CoV-2Nucleoprotein antibody (A21042) at 1:10000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 0.2s.

ABclonal:Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)

Immunofluorescence analysis of 293T-N and 293T using SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	SARS-CoV-2 Nucleoprotein Rabbit mAb
Catalog No.	A21042
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC51499

Background

Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an enveloped, positive-sense, single-stranded RNA virus that causes coronavirus disease 2019 (COVID-19). Virus particles include the RNA genetic material and structural proteins needed for invasion of host cells. Once inside the cell the infecting RNA is used to encode structural proteins that make up virus particles, nonstructural proteins that direct virus assembly, transcription, replication and host control and accessory proteins whose function has not been determined.~ The structural proteins of SARS-CoV-2 include the envelope protein (E), spike or surface glycoprotein (S), membrane protein (M) and the nucleocapsid protein (N). The nucleocapsid phosphoprotein is a structural protein that binds to, protects the viral RNA genome and is involved in packaging the RNA into virus particles. The N protein has been suggested as an antiviral drug target.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-420 of coronavirus Nucleoprotein (P0DTC9).
Sequence	MSDNGPQNQRNAPRITFGGPSDSTGSNQNGERSGARSKQRRPQGLPNNTASWFTALTQHGKEDLKFPRGQGVPINTNSSPDDQIGYYRRATRRIRGGDGKMKDLSPRWYFYYLGTGPEAGLPYGANKDGIIWVATEGALNTPKDHIGTRNPANNAAIVLQLPQGTTLPKGFYAEGSRGGSQASSRSSSRSRNSSRNSTPGSSRGTSPARMAGNGGDAALALLLLDRLNQLESKMSGKGQQQQGQTVTKKSAAEASKKPRQKRTATKAYNVTQAFGRRGPEQTQGNFGDQELIRQGTDYKHWPQIAQFAPSASAFFGMSRIGMEVTPSGTWLTYTGAIKLDDKDPNFKDQVILLNKHIDAYKTFPPTEPKKDKKKKADETQALPQRQKKQQTVTLLPAADLDDFSKQLQQSMSSADSTQA
Gene ID
Swiss Prot
Synonyms	N; Nucleoprotein; NC;
Calculated MW	46kDa
Observed MW	50kDa

Applications

Reactivity	SARS-CoV-2
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:2000 - 1:10000 IF/ICC 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HEK-293T (human embryonic kidney) transfected with SARS-CoV-2 Nucleocapsid protein
Cellular location	Virion, Host cytoplasm, Secreted, Host extracellular space;
Customer validation	IHC(Homo sapiens) WB(Mus musculus)

Documents

Certificate of Compliance

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Research Area

SARS-CoV-2 Nucleoprotein Rabbit mAb images

Western blot - SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)

Immunofluorescence - SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042)

Immunofluorescence analysis of 293T-N and 293T using SARS-CoV-2 Nucleoprotein Rabbit mAb (A21042) at dilution of 1:400 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.