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ABflo® 405-conjugated Goat anti-Mouse IgG (H+L) (AS055)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Flow CytoMetry - ABflo® 405-conjugated Goat anti-Mouse IgG (H+L) (AS055)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Hep G2 cells (right) were surface-stained with Mouse Anti-Human GPC3 mAb (4 μg/mL, orange line) or secondary antibody only (blue line). Non-fluorescently stained Hep G2 and K-562 cells were used as blank control (red line). ABflo® 405-conjugated Goat Anti-Mouse IgG (H+L)(AS055, 1:1000) was used as a secondary antibody.

Overview

Product nameABflo® 405-conjugated Goat anti-Mouse IgG (H+L)
Catalog No.AS055
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • FC 1:500 - 1:2000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationFlow Cytometry    
Positive samples
Cellular location
Customer validation

WB(Homo sapiens)

IF(Other)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Flow CytoMetry - ABflo® 405-conjugated Goat anti-Mouse IgG (H+L) (AS055)}

    Flow CytoMetry - ABflo® 405-conjugated Goat anti-Mouse IgG (H+L) (AS055)

    Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Hep G2 cells (right) were surface-stained with Mouse Anti-Human GPC3 mAb (4 μg/mL, orange line) or secondary antibody only (blue line). Non-fluorescently stained Hep G2 and K-562 cells were used as blank control (red line). ABflo® 405-conjugated Goat Anti-Mouse IgG (H+L)(AS055, 1:1000) was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS055? Please let us know so that we can cite the reference in this datasheet.