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HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)

Publications (4) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Western blot - HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)

Western blot analysis of recombinant Mouse control IgG Protein using HRP Donkey Anti-Mouse IgG (H+L)(AS033) at 1:10000 dilution.
Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L)(AS033) at 1:10000 dilution.
Lysates/proteins: 100ng/50ng per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Western blot - HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)

Western blot analysis of lysates from HeLa cells, using GAPDH antibody as the primary antibody.
Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L)(AS033) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 20s.

ABclonal:Immunohistochemistry - HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)

Immunohistochemistry analysis of paraffin-embedded Mouse brain using HRP Donkey Anti-Mouse IgG (H+L) (AS033) at dilution of 1:20 - 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

Overview

Product nameHRP-conjugated Donkey anti-Mouse IgG (H+L)
Catalog No.AS033
Host speciesDonkey
Purification methodAffinity purification
IsotypeHorseradish peroxidase conjugated IgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW25kDa(Light chain), 55kDa(Heavy chain)/36kDa
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:10000 - 1:30000
  • IHC-P 1:500 - 1:1000
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.75% BSA, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesMouse control IgG, HeLa
Cellular location
Customer validation

WB(Mus musculus, Homo sapiens)

Cell-based assay(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)}

    Western blot - HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)

    Western blot analysis of recombinant Mouse control IgG Protein using HRP Donkey Anti-Mouse IgG (H+L)(AS033) at 1:10000 dilution.
    Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L)(AS033) at 1:10000 dilution.
    Lysates/proteins: 100ng/50ng per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.

    ABclonal:Western blot - HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)}

    Western blot - HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)

    Western blot analysis of lysates from HeLa cells, using GAPDH antibody as the primary antibody.
    Secondary antibody:HRP Donkey Anti-Mouse IgG (H+L)(AS033) at 1:10000 dilution.
    Lysates/proteins: 25 μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 20s.
    ABclonal:Immunohistochemistry - HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)}

    Immunohistochemistry - HRP-conjugated Donkey anti-Mouse IgG (H+L) (AS033)

    Immunohistochemistry analysis of paraffin-embedded Mouse brain using HRP Donkey Anti-Mouse IgG (H+L) (AS033) at dilution of 1:20 - 1:800 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS033? Please let us know so that we can cite the reference in this datasheet.