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Phospho-PTEN-S380/T382/T383 Rabbit mAb (AP1346)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates, using Phospho-PTEN-S380/T382/T383 Rabbit mAb (AP1346) at1:2000 dilution.Hela, NIH/3T3, and PC-12 cells were treated by CIP(20uL/400ul) at 37℃ for 1 hour.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunohistochemistry - Phospho-PTEN-S380/T382/T383 Rabbit mAb (AP1346)

Immunohistochemistry analysis of Phospho-PTEN-S380/T382/T383 in paraffin-embedded rat lung tissue using Phospho-PTEN-S380/T382/T383 Rabbit mAb (AP1346) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Phospho-PTEN-S380/T382/T383 in paraffin-embedded mouse colon tissue using Phospho-PTEN-S380/T382/T383 Rabbit mAb (AP1346) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Phospho-PTEN-S380/T382/T383 in paraffin-embedded human cervix cancer tissue using Phospho-PTEN-S380/T382/T383 Rabbit mAb (AP1346) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of Phospho-PTEN-S380/T382/T383 in paraffin-embedded Human lung adenocarcinoma tissue using Phospho-PTEN-S380/T382/T383 Rabbit mAb (AP1346) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-PTEN-S380/T382/T383 Rabbit mAb (AP1346)

Confocal imaging of paraffin-embedded Rat brain tissue using Phospho-PTEN-S380/T382/T383 Rabbit mAb (AP1346, dilution 1:200) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). DAPI was used for nuclear staining (Blue). Objective: 40x. Perform microwave antigen retrieval with 0.01 M citrate buffer (pH 6.0) prior to IF staining.

Overview

Product name	Phospho-PTEN-S380/T382/T383 Rabbit mAb
Catalog No.	AP1346
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC55940

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S380 & T383 of human Phospho-PTEN-S380/T382/T383 (NP_000305.3).
Sequence	YSDTTD
Gene ID
Swiss Prot
Synonyms	BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta; Phospho-PTEN-S380/T382/T383
Calculated MW	47kDa
Observed MW	54kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	Hela, NIH/3T3, PC-12
Cellular location	Apical plasma membrane, Cell projection, Cytoplasm, Cytoplasmic side of plasma membrane, Cytosol, Dendritic spine, Extracellular region, Myelin sheath adaxonal region, Neuron projection, Nucleoplasm, Nucleus, plasma membrane, PML body, Postsynaptic density
Customer validation	WB(Homo sapiens)