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Phospho-PP2A Catalytic α-Y307 Rabbit mAb (AP1043)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-PP2A Catalytic α-Y307 Rabbit mAb (AP1043) at 1:1000 dilution. NIH/3T3 cells were treated by Forskolin (30 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3s.

Overview

Product name	Phospho-PP2A Catalytic α-Y307 Rabbit mAb
Catalog No.	AP1043
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC1589

Background

This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around Y307 of human PP2A Catalytic α (P67775).
Sequence	PDYFL
Gene ID
Swiss Prot
Synonyms	RP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha; Phospho-PP2A Catalytic α-Y307
Calculated MW	36kDa
Observed MW	35kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	NIH/3T3 treated by Forskolin
Cellular location	Chromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, spindle pole.
Customer validation	WB(Sus scrofa, Malus pumila)