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PP2A Catalytic α Rabbit pAb (A6702)

Review (1)Publications (6) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - PP2A Catalytic α Rabbit pAb (A6702)

Western blot analysis of various lysates, using PP2A Catalytic α Rabbit pAb (A6702) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

ABclonal:Immunohistochemistry - PP2A Catalytic α Rabbit pAb (A6702)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using PP2A Catalytic α Rabbit pAb (A6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PP2A Catalytic α Rabbit pAb (A6702)

Immunohistochemistry analysis of paraffin-embedded Human cervical cance tissue using PP2A Catalytic α Rabbit pAb (A6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunohistochemistry - PP2A Catalytic α Rabbit pAb (A6702)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PP2A Catalytic α Rabbit pAb (A6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - PP2A Catalytic α Rabbit pAb (A6702)

Immunofluorescence analysis of HeLa cells using PP2A Catalytic α Rabbit pAb (A6702) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunofluorescence - PP2A Catalytic α Rabbit pAb (A6702)

Immunofluorescence analysis of NIH/3T3 cells using PP2A Catalytic α Rabbit pAb (A6702) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - PP2A Catalytic α Rabbit pAb (A6702)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg PP2A Catalytic α antibody (A6702). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (A6702) at a dilution of 1:1000.

ABclonal:Immunoprecipitation - PP2A Catalytic α Rabbit pAb (A6702)

Immunoprecipitation analysis of 500 μg extracts of Rat brain cells using 3 μg PP2A Catalytic α antibody (A6702). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (A6702) at a dilution of 1:1000.

Review
ABclonal: review for PP2A Catalytic α Rabbit pAb(A6702)

Overview

Product namePP2A Catalytic α Rabbit pAb
Catalog No.A6702
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes the phosphatase 2A catalytic subunit. Protein phosphatase 2A is one of the four major Ser/Thr phosphatases, and it is implicated in the negative control of cell growth and division. It consists of a common heteromeric core enzyme, which is composed of a catalytic subunit and a constant regulatory subunit, that associates with a variety of regulatory subunits. This gene encodes an alpha isoform of the catalytic subunit.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-309 of human PP2A Catalytic α (NP_002706.1).
SequenceMDEKVFTKELDQWIEQLNECKQLSESQVKSLCEKAKEILTKESNVQEVRCPVTVCGDVHGQFHDLMELFRIGGKSPDTNYLFMGDYVDRGYYSVETVTLLVALKVRYRERITILRGNHESRQITQVYGFYDECLRKYGNANVWKYFTDLFDYLPLTALVDGQIFCLHGGLSPSIDTLDHIRALDRLQEVPHEGPMCDLLWSDPDDRGGWGISPRGAGYTFGQDISETFNHANGLTLVSRAHQLVMEGYNWCHDRNVVTIFSAPNYCYRCGNQAAIMELDDTLKYSFLQFDPAPRRGEPHVTRRTPDYFL
Gene ID
Swiss Prot
SynonymsRP-C; PP2Ac; PP2CA; NEDLBA; PP2Calpha; PP2A Catalytic α
Calculated MW36kDa
Observed MW36kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:1000 - 1:5000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
  • IP 0.5μg-4μg antibody for 200μg-500μg extracts of whole cells
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    Immunoprecipitation    
Positive samplesHeLa, 293T, NIH/3T3, Rat brain
Cellular locationChromosome, Cytoplasm, Nucleus, centromere, cytoskeleton, spindle pole.
Customer validation

(143B,MNNG)

WB(Mus musculus, Sus scrofa, Homo sapiens, Penaeus japonicus)

IHC(Mus musculus)

Documents

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ABclonal:Western blot - PP2A Catalytic α Rabbit pAb (A6702)}

Western blot - PP2A Catalytic α Rabbit pAb (A6702)

Western blot analysis of various lysates, using PP2A Catalytic α Rabbit pAb (A6702) at 1:2000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
ABclonal:Immunohistochemistry - PP2A Catalytic α Rabbit pAb (A6702)}

Immunohistochemistry - PP2A Catalytic α Rabbit pAb (A6702)

Immunohistochemistry analysis of paraffin-embedded Mouse heart tissue using PP2A Catalytic α Rabbit pAb (A6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PP2A Catalytic α Rabbit pAb (A6702)}

Immunohistochemistry - PP2A Catalytic α Rabbit pAb (A6702)

Immunohistochemistry analysis of paraffin-embedded Human cervical cance tissue using PP2A Catalytic α Rabbit pAb (A6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunohistochemistry - PP2A Catalytic α Rabbit pAb (A6702)}

Immunohistochemistry - PP2A Catalytic α Rabbit pAb (A6702)

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using PP2A Catalytic α Rabbit pAb (A6702) at a dilution of 1:100 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.
ABclonal:Immunofluorescence - PP2A Catalytic α Rabbit pAb (A6702)}

Immunofluorescence - PP2A Catalytic α Rabbit pAb (A6702)

Immunofluorescence analysis of HeLa cells using PP2A Catalytic α Rabbit pAb (A6702) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunofluorescence - PP2A Catalytic α Rabbit pAb (A6702)}

Immunofluorescence - PP2A Catalytic α Rabbit pAb (A6702)

Immunofluorescence analysis of NIH/3T3 cells using PP2A Catalytic α Rabbit pAb (A6702) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.
ABclonal:Immunoprecipitation - PP2A Catalytic α Rabbit pAb (A6702)}

Immunoprecipitation - PP2A Catalytic α Rabbit pAb (A6702)

Immunoprecipitation analysis of 200 μg extracts of 293T cells using 3 μg PP2A Catalytic α antibody (A6702). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (A6702) at a dilution of 1:1000.
ABclonal:Immunoprecipitation - PP2A Catalytic α Rabbit pAb (A6702)}

Immunoprecipitation - PP2A Catalytic α Rabbit pAb (A6702)

Immunoprecipitation analysis of 500 μg extracts of Rat brain cells using 3 μg PP2A Catalytic α antibody (A6702). Western blot was performed from the immunoprecipitate using PP2A Catalytic α antibody (A6702) at a dilution of 1:1000.

* For research use only. Not for therapeutic or diagnostic purposes.

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