제품 > 항체 > 다중클론항체(pAb)

AMPKa1/AMPKa2 Rabbit pAb (A17290)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of 293F, using AMPKa1/AMPKa2 antibody (A17290) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A17290)

Immunofluorescence analysis of NIH/3T3 cells using AMPKa1/AMPKa2 Rabbit pAb (A17290) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of PC-12 cells using AMPKa1/AMPKa2 Rabbit pAb (A17290) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U2OS cells using AMPKa1/AMPKa2 Rabbit pAb (A17290) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Overview

Product name	AMPKa1/AMPKa2 Rabbit pAb
Catalog No.	A17290
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 5-280 of human AMPKa1/AMPKa2 (NP_006242.5).
Sequence	SSWRKMATAEKQKHDGRVKIGHYILGDTLGVGTFGKVKVGKHELTGHKVAVKILNRQKIRSLDVVGKIRREIQNLKLFRHPHIIKLYQVISTPSDIFMVMEYVSGGELFDYICKNGRLDEKESRRLFQQILSGVDYCHRHMVVHRDLKPENVLLDAHMNAKIADFGLSNMMSDGEFLRTSCGSPNYAAPEVISGRLYAGPEVDIWSSGVILYALLCGTLPFDDDHVPTLFKKICDGIFYTPQYLNPSVISLLKHMLQVDPMKRATIKDIREHEWFK
Gene ID
Swiss Prot
Synonyms	AMPKa1/AMPKa2
Calculated MW	64kDa/65kDa
Observed MW	62kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	293F
Cellular location	apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens)

Documents

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Research Area

AMPKa1/AMPKa2 Rabbit pAb images

Western blot - AMPKa1/AMPKa2 Rabbit pAb (A17290)

Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A17290)

Immunofluorescence analysis of NIH/3T3 cells using AMPKa1/AMPKa2 Rabbit pAb (A17290) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A17290)

Immunofluorescence analysis of PC-12 cells using AMPKa1/AMPKa2 Rabbit pAb (A17290) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

Immunofluorescence - AMPKa1/AMPKa2 Rabbit pAb (A17290)

Immunofluorescence analysis of U2OS cells using AMPKa1/AMPKa2 Rabbit pAb (A17290) at dilution of 1:50 (40x lens). Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for AMPKa1/AMPKa2 Rabbit pAb (A17290)

Product:

AMPKa1/AMPKa2 Rabbit pAb

Journal:

The EMBO Journal

Application:

IF:

11.598

Species:

Mus musculus

PMID:

32104923

Title:

Exercise-induced α-ketoglutaric acid stimulates muscle hypertrophy and fat loss through OXGR1-dependent adrenal activation

References for AMPKa1/AMPKa2 Rabbit pAb (A17290)

Product:

AMPKa1/AMPKa2 Rabbit pAb

Journal:

Phytomedicine

Application:

IF:

5.34

Species:

Rattus norvegicus

PMID:

34333328

Title:

Berberine improves ovulation and endometrial receptivity in polycystic ovary syndrome

References for AMPKa1/AMPKa2 Rabbit pAb (A17290)

Product:

AMPKa1/AMPKa2 Rabbit pAb

Journal:

Nutrients

Application:

IF:

6.7

Species:

Mus musculus

PMID:

37447331

Title:

Dihydro-Resveratrol Attenuates Oxidative Stress, Adipogenesis and Insulin Resistance in In Vitro Models and High-Fat Diet-Induced Mouse Model via AMPK …

References for AMPKa1/AMPKa2 Rabbit pAb (A17290)

Product:

AMPKa1/AMPKa2 Rabbit pAb

Journal:

Journal of Translational Medicine

Application:

IF:

7.4

Species:

Homo sapiens

PMID:

37633909

Title:

Glutathione Peroxidase 3 induced mitochondria-mediated apoptosis via AMPK /ERK1/2 pathway and resisted autophagy-related ferroptosis via AMPK/mTOR pathway in hyperplastic prostate

References for AMPKa1/AMPKa2 Rabbit pAb (A17290)

Product:

AMPKa1/AMPKa2 Rabbit pAb

Journal:

3 Biotech

Application:

IF:

2.89

Species:

Rattus norvegicus

PMID:

38125651

Title:

Thymoquinone mitigates obesity and diabetic parameters through regulation of major adipokines, key lipid metabolizing enzymes and AMPK/p-AMPK in diet-induced

References for AMPKa1/AMPKa2 Rabbit pAb (A17290)

Product:

AMPKa1/AMPKa2 Rabbit pAb

Journal:

Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapie

Application:

IF:

7.41

Species:

Rattus norvegicus

PMID:

35856853