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Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (AP1171)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb (AP1171) at 1:1000 dilution. 293T cells were treated by oligomycin (0. 5 uM) at 37℃ for 30 minutes. C2C12 cells were treated by oligomycin (0. 5 uM) at 37℃ for 30 minutes. C6 cells were treated by oligomycin (0. 5 uM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% BSA.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 60s.

Overview

Product name	Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb
Catalog No.	AP1171
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T183 of human AMPKa1/AMPKa2PRKAA1 (NP_006242.5).
Sequence	LRTSC
Gene ID
Swiss Prot
Synonyms	AMPKa1/AMPKa2; Phospho-AMPKa1/AMPKa2-T183/T172
Calculated MW	64kDa/65kDa/62kDa
Observed MW	62kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T treated by oligomycin, C2C12 treated by oligomycin, C6 treated by oligomycin
Cellular location	apical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus.
Customer validation	WB(Mus musculus, Rattus norvegicus, Homo sapiens) IHC(Rattus norvegicus) IF(Rattus norvegicus) ELISA(Rattus norvegicus)