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Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)

Publications (15) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)

Western blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432) at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Enhanced Kit (RM00021).
Exposure time: 90s.

ABclonal:Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)

Western blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432) at 1:1000 dilution. C6 cells were treated by AICAR (0.5 mM) at 37℃ for 30 minutes after serum-starvation overnight.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)

Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432) at 1:400 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product namePhospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb
Catalog No.AP0432
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed.
ImmunogenA synthetic phosphorylated peptide around T183 of human PRKAA1 (NP_006242.5).
SequenceLRTSC
Gene ID
Swiss Prot
SynonymsAMPKa1/AMPKa2; Phospho-AMPKa1-T183/AMPKa2-T172
Calculated MW64kDa/65kDa/62kDa
Observed MW64kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    
Positive samplesC6 treated by AICAR
Cellular locationapical plasma membrane, cytoplasm, cytosol, nuclear speck, nucleoplasm, nucleus.
Customer validation

IHC(Mus musculus)

WB(Rattus norvegicus, Mus musculus, Sus scrofa, Homo sapiens, Mus musculus, ovis aries)

IF(Sus scrofa)

IF(Mus musculus)

WB(Mus musculus)

Documents

Certificate of Compliance

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Lot number

    ABclonal:Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)}

    Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)

    Western blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432) at 1:1000 dilution. C6 cells were treated by AICAR (0. 5 mM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Enhanced Kit (RM00021).
    Exposure time: 90s.
    ABclonal:Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)}

    Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)

    Western blot analysis of lysates from C6 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432) at 1:1000 dilution. C6 cells were treated by AICAR (0.5 mM) at 37℃ for 30 minutes after serum-starvation overnight.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 90s.
    ABclonal:Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)}

    Western blot - Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432)

    Western blot analysis of lysates from C2C12 cells, using Phospho-AMPKa1-T183/AMPKa2-T172 Rabbit pAb (AP0432) at 1:400 dilution. C2C12 cells were treated by oligomycin (0. 5 uM) at 37℃ for 30 minutes.
    Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
    Lysates/proteins: 25μg per lane.
    Blocking buffer: 3% nonfat dry milk in TBST.
    Detection: ECL Basic Kit (RM00020).
    Exposure time: 180s.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AP0432? Please let us know so that we can cite the reference in this datasheet.

    항체 (6)

    Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb
    AP0116

    WBIHC-PIPELISA

    Reactivity:Human, Mouse, Rat

    Phospho-AMPKα1-T183/AMPKα2-T172 Rabbit pAb

    AMPKa1/AMPKa2 Rabbit pAb
    A12718

    WBIHC-PIF/ICCELISA

    Reactivity:Human, Mouse, Rat

    AMPKa1/AMPKa2 Rabbit pAb

    AMPKa1/AMPKa2 Rabbit pAb
    A17290

    ELISAWBIF/ICC

    Reactivity:Human, Mouse, Rat

    AMPKa1/AMPKa2 Rabbit pAb

    Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb
    AP1171

    ELISAWB

    Reactivity:Human, Mouse, Rat

    Phospho-AMPKa1/AMPKa2-T183/T172 Rabbit pAb

    Phospho-AMPKα1-T183+AMPKα2-T172 Rabbit mAb
    AP1441

    ELISAWB

    Reactivity:Human, Mouse, Rat

    Phospho-AMPKα1-T183+AMPKα2-T172 Rabbit mAb

    AMPKa1/AMPKa2 Mouse mAb
    A27099

    WBIHC-PELISA

    Reactivity:Human, Mouse, Rat

    AMPKa1/AMPKa2 Mouse mAb

    Secondary Antibodies (26)

    HRP-conjugated Goat anti-Rabbit IgG (H+L)
    AS014

    WBDBIHC-PELISA

    HRP-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Goat anti-Rabbit IgG (H+L)
    AS011

    IF/ICCFC

    FITC-conjugated Goat anti-Rabbit IgG (H+L)

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)
    AS007

    IF/ICCFC

    Cy3-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS053

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain
    AS061

    WBIP

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain
    AS063

    WB

    HRP-conjugated Goat anti-Rabbit IgG Heavy Chain

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)
    AS039

    IF/ICCFC

    ABflo® 594-conjugated Goat anti-Rabbit IgG (H+L)

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)
    AS040

    IHC-PIF/ICCFC

    Rhodamine (TRITC)-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)
    AS060

    IF/ICCFC

    ABflo® 647-conjugated Goat anti-Rabbit IgG (H+L)

    Goat Anti-Rabbit IgG(H+L)
    AS070

    WBELISACUT&Tag

    Goat Anti-Rabbit IgG(H+L)

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)
    AS038

    WBIHC-P

    HRP-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)
    AS035

    IF/ICCFC

    ABflo® 488-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)
    AS073

    IF/ICCFC

    ABflo® 488-conjugated Goat anti-Rabbit IgG (H+L)

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)
    AS058

    IF/ICCFC

    ABflo® 555-conjugated Goat anti-Rabbit IgG (H+L)

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)
    AS042

    IF/ICCFC

    FITC-conjugated Donkey anti-Rabbit IgG (H+L)

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)
    AS056

    IF/ICCFC

    ABflo® 405-conjugated Goat anti-Rabbit IgG (H+L)

    AP-conjugated Goat anti-Rabbit IgG (H+L)
    AS009

    WB

    AP-conjugated Goat anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)
    AS079

    WB

    HRP-conjugated Mouse anti-Rabbit IgG (H+L)

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb
    AS126

    IPELISA

    HRP-conjugated Mouse anti-Rabbit IgG Light Chain mAb

    PE Mouse anti-Rabbit IgG, light chain specific mAb
    AS129

    FC

    PE Mouse anti-Rabbit IgG, light chain specific mAb

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb
    AS122

    WBELISA

    HRP conjugated Mouse anti-Rabbit IgG Heavy Chain mAb

    Rabbit Anti-Chicken IgY (H+L)
    AS090

    WB

    Rabbit Anti-Chicken IgY (H+L)

    Rabbit Anti-Chicken IgY Fab
    AS088

    WB

    Rabbit Anti-Chicken IgY Fab

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific
    AS083

    IF/ICCFC

    FITC-conjugated F(ab')₂ Fragment Goat anti-Rabbit IgG, Fc fragment specific

    Rabbit Anti-Chicken IgY (H+L)
    AS082

    WB

    Rabbit Anti-Chicken IgY (H+L)

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)
    AS072

    IF/ICCFC

    ABflo® 750-conjugated Goat anti-Rabbit IgG (H+L)