Recombinant Human Vimentin/VIM Protein (RP03179)

Overview

Product name	Recombinant Human Vimentin/VIM Protein
Catalog No.	RP03179
Description	Recombinant Human Vimentin/VIM Protein is produced by Baculovirus-Insect Cells expression system. The target protein is expressed with sequence (Met1-Glu466) of Human Vimentin/VIM (Accession #NP_003371) fused with a His tag at the C-terminus.
Purity	> 95% by SDS-PAGE.
Endotoxin	< 1 EU/μg
Formulation	Lyophilized from a 0.22 μm filtered solution of 0.1% TFA, 40% acetonitrile.
Species	Human
Calculated MW	55.11 KDa.

Vimentin is a type III intermediate filament (IF) protein found in various non-epithelial cells, especially mesenchymal cells. A vimentin monomer, has a central α-helical domain and carboxyl (tail) domains. Two monomers compose the basic subunit of vimentin assembly. Vimentin is crucial for supporting and anchoring the position of the organelles in the cytosol. Vimentin provided cells with a resilience absent from the microtubule or actin filament networks, when under mechanical stress in vivo. Therefore, in general, it is accepted that vimentin is the cytoskeletal component responsible for maintaining cell integrity. Vimentin is also responsible for stabilizing cytoskeletal interactions. It is found that vimentin control the transport of low-density lipoprotein. It has been used as a sarcoma tumor marker to identify mesenchyme.

Product information

Synonym	CTRCT30; HEL113; Vimentin
Expression Host	Baculovirus-Insect Cells
Tag	C-His
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.