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Phospho-Vimentin-S83 Rabbit pAb (AP1119)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-Vimentin-S83 Rabbit pAb (AP1119) at 1:1000 dilution. NIH/3T3 cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. NIH/3T3 cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from C6 cells, using Phospho-Vimentin-S83 Rabbit pAb (AP1119) at 1:1000 dilution. C6 cells were treated by Paclitaxel (100 nM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from HeLa cells, using Phospho-Vimentin-S83 Rabbit pAb (AP1119) at 1:1000 dilution. HeLa cells were treated by Paclitaxel (100 nM/ml) at 37℃ for 20 hours. HeLa cells were treated by Hydroxyurea (4 mM) at 37℃ for 20 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Phospho-Vimentin-S83 Rabbit pAb
Catalog No.	AP1119
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S83 of human VIM (NP_003371.2).
Sequence	QDSVD
Gene ID
Swiss Prot
Synonyms	VIM; CTRCT30; HEL113; vimentin; Phospho-Vimentin-S83
Calculated MW	54kDa
Observed MW	57kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by Paclitaxel, HeLa treated by Hydroxyurea, NIH/3T3 treated by Paclitaxel, NIH/3T3 treated by Hydroxyurea , C6 treated by Paclitaxel
Cellular location	Cytoplasm.