제품 > 항체 > 다중클론항체(pAb)

[KD Validated] Vimentin Rabbit pAb (A11952)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KD Validated] Vimentin Rabbit pAb (A11952) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of lysates from wild type (WT) and Vimentin knockdown (KD) 293T cells, using [KD Validated] Vimentin Rabbit pAb (A11952) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 5s.

ABclonal:Immunohistochemistry - [KD Validated] Vimentin Rabbit pAb (A11952)

Immunohistochemistry analysis of paraffin-embedded Human colon using Vimentin Rabbit pAb (A11952) at dilution of 1:100 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] Vimentin Rabbit pAb (A11952)

Immunofluorescence analysis of U2OS cells using [KD Validated] Vimentin Rabbit pAb (A11952) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Review

Overview

Product name	[KD Validated] Vimentin Rabbit pAb
Catalog No.	A11952
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a type III intermediate filament protein. Intermediate filaments, along with microtubules and actin microfilaments, make up the cytoskeleton. The encoded protein is responsible for maintaining cell shape and integrity of the cytoplasm, and stabilizing cytoskeletal interactions. This protein is involved in neuritogenesis and cholesterol transport and functions as an organizer of a number of other critical proteins involved in cell attachment, migration, and signaling. Bacterial and viral pathogens have been shown to attach to this protein on the host cell surface. Mutations in this gene are associated with congenital cataracts in human patients.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human Vimentin (NP_003371.2).
Sequence	MSTRSVSSSSYRRMFGGPGTASRPSSSRSYVTTSTRTYSLGSALRPSTSRSLYASSPGGVYATRSSAVRLRSSVPGVRLLQDSVDFSLADAINTEFKNTRT
Gene ID
Swiss Prot
Synonyms	CTRCT30; HEL113; Vimentin; VIM; vimentin; in
Calculated MW	54kDa
Observed MW	57kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293T, Jurkat, NIH/3T3, C6, Mouse testis
Cellular location	Cytoplasm.
Customer validation	(Human glioma cells) WB(Homo sapiens, Mus musculus, Canis lupus familiaris, Mus musculus, Rattus norvegicus, Other) IF(Homo sapiens) IHC(Homo sapiens, Mus musculus) ELISA(Homo sapiens, Mus musculus, Rattus norvegicus) IHC(Homo sapiens) IF(Homo sapiens) FC(Homo sapiens) IF(Homo sapiens) WB(Homo sapiens)

Documents

Certificate of Compliance

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Lot number

Research Area

[KD Validated] Vimentin Rabbit pAb images

Western blot - [KD Validated] Vimentin Rabbit pAb (A11952)

Immunohistochemistry - [KD Validated] Vimentin Rabbit pAb (A11952)

Immunofluorescence - [KD Validated] Vimentin Rabbit pAb (A11952)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A11952

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Reviews List

Product Protocols

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Journal of Experimental & Clinical Cancer Research

Application:

IF:

11.161

Species:

Homo sapiens

PMID:

29197410

Title:

Direct inhibition of ACTN4 by ellagic acid limits breast cancer metastasis via regulation of β-catenin stabilization in cancer stem cells

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Scientific reports

Application:

IF:

4.379

Species:

Homo sapiens

PMID:

29109519

Title:

Network-pharmacology-based validation of TAMS/CXCL-1 as key mediator of XIAOPI formula preventing breast cancer development and metastasis

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Toxicology and applied pharmacology

Application:

IF:

4.219

Species:

Homo sapiens

PMID:

31381904

Title:

The involvement of lipid raft pathway in suppression of TGFβ-mediated metastasis by tolfenamic acid in hepatocellular carcinoma cells.

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Journal of Cellular Biochemistry

Application:

IF:

4.429

Species:

Homo sapiens

PMID:

31696971

Title:

SNHG16 as the miRNA let-7b-5p sponge facilitates the G2/M and epithelial-mesenchymal transition by regulating CDC25B and HMGA2 expression in hepatocellular carcinoma

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

8.469

Species:

Homo sapiens

PMID:

31511502

Title:

SNRPB promotes the tumorigenic potential of NSCLC in part by regulating RAB26.

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Pharmacol Res

Application:

IF:

7.658

Species:

Mus musculus

PMID:

32492489

Title:

Oroxylin A suppresses ACTN1 expression to inactivate cancer-associated fibroblasts and restrain breast cancer metastasis

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Dev Cell

Application:

IF:

12.27

Species:

Homo sapiens

PMID:

33238152

Title:

A Single-Cell Transcriptomic Atlas of Human Skin Aging

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Drug Dev Res

Application:

IF:

4.36

Species:

Homo sapiens

PMID:

33112006

Title:

Bergenin inhibits bladder cancer progression via activating the PPARγ/PTEN/Akt signal pathway

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Drug Des Devel Ther

Application:

IF:

4.16

Species:

Homo sapiens

PMID:

33833498

Title:

Chlorogenic Acid Inhibits Human Glioma U373 Cell Progression via Regulating the SRC/MAPKs Signal Pathway: Based on Network Pharmacology Analysis

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Biochem Biophys Res Commun

Application:

IF:

3.575

Species:

Homo sapiens

PMID:

33631676

Title:

FLOT2 upregulation promotes growth and invasion by interacting and stabilizing EphA2 in gliomas

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Transl Lung Cancer Res

Application:

IF:

6.49

Species:

Homo sapiens

PMID:

33889522

Title:

circFBXW7 attenuates malignant progression in lung adenocarcinoma by sponging miR-942-5p

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Pharmacol Res

Application:

WB, IHC

IF:

7.65

Species:

Homo sapiens

PMID:

34450313

Title:

Methyltransferase-like protein 11A promotes migration of cervical cancer cells via up-regulating ELK3

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Mol Ther Nucleic Acids

Application:

IF:

8.88

Species:

Mus musculus

PMID:

32585629

Title:

miR-301a Suppression within Fibroblasts Limits the Progression of Fibrosis through the TSC1/mTOR Pathway

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

4.55

Species:

Mus musculus

PMID:

35055127

Title:

Placenta-Specific miR-125b Overexpression Leads to Increased Rates of Pregnancy Loss in Mice

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Cancers

Application:

IF:

Species:

Homo sapiens

PMID:

34503284

Title:

Targeting S1PR1 May Result in Enhanced Migration of Cancer Cells in Bladder Carcinoma

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Journal of cancer research and clinical oncology

Application:

IF:

4.32

Species:

Homo sapiens

PMID:

35960375

Title:

Cannabidiol inhibits invasion and metastasis in colorectal cancer cells by reversing epithelial–mesenchymal transition through the Wnt/β-catenin signaling pathway

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Homo sapiens

PMID:

35927229

Title:

Up-regulation of SLC27A2 suppresses the proliferation and invasion of renal cancer by down-regulating CDK3-mediated EMT - PMC

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Frontiers in cell and developmental biology

Application:

IF:

6.08

Species:

Canis lupus familiaris

PMID:

36760362

Title:

Effect of cold atmospheric microwave plasma (CAMP) on wound healing in canine keratinocytes - PMC

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Current medical science

Application:

IF:

2.64

Species:

Homo sapiens

PMID:

36544038

Title:

Dysregulated RUNX1 Predicts Poor Prognosis by Mediating Epithelialmesenchymal Transition in Cervical Cancer

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

36590170

Title:

SLC17A9-PTHLH-EMT axis promotes proliferation and invasion of clear renal cell carcinoma - PMC

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

ERJ open research

Application:

IF:

4.23

Species:

Homo sapiens

PMID:

37260462

Title:

The effects of dual IQOS and cigarette smoke exposure on airway epithelial cells: implications for lung health and respiratory disease pathogenesis - PMC

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.92

Species:

Homo sapiens

PMID:

37836626

Title:

Bufotalin Suppresses Proliferation and Metastasis of Triple-Negative Breast Cancer Cells by Promoting Apoptosis and Inhibiting the STAT3/EMT Axis - PMC

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Advanced science (Weinheim, Baden-Wurttemberg, Germany)

Application:

WB, ELISA

IF:

17.52

Species:

Homo sapiens, Mus musculus, Rattus norvegicus

PMID:

37566765

Title:

Nuclear Actin Polymerization Regulates Cell EpithelialMesenchymal Transition

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Cell Death Discov

Application:

IF:

7.109

Species:

Homo sapiens, Mus musculus

PMID:

34759267

Title:

Small nucleolar RNA 42 promotes the growth of hepatocellular carcinoma through the p53 signaling pathway

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Theranostics

Application:

IF:

11.6

Species:

Homo sapiens

PMID:

32929380

Title:

circPTCH1 promotes invasion and metastasis in renal cell carcinoma via regulating miR-485-5p/MMP14 axis

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Cell Death Dis

Application:

IHC, IF

IF:

9.685

Species:

Homo sapiens

PMID:

33087705

Title:

RPN2 is targeted by miR-181c and mediates glioma progression and temozolomide sensitivity via the wnt/-catenin signaling pathway

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Pharmaceutics

Application:

IF:

6.52

Species:

Homo sapiens

PMID:

36986805

Title:

A Novel Fibromodulin Antagonist Peptide RP4 Exerts Antitumor Effects on Colorectal Cancer - PMC

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Biochemical pharmacology

Application:

WB, IHC

IF:

6.1

Species:

Homo sapiens, Mus musculus

PMID:

37558004

Title:

N-3, a novel synthetic derivative of bifendate, inhibits metastasis of triple-negative breast cancer via decreasing p38-regulated FOXC1 protein stability

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Translational oncology

Application:

IF:

4.8

Species:

Homo sapiens

PMID:

38039946

Title:

miR-1182-mediated ALDH3A2 inhibition affects lipid metabolism and progression in ccRCC by activating the PI3K-AKT pathway

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Pathology, research and practice

Application:

IF:

3.3

Species:

Homo sapiens,Mus musculus

PMID:

38237399

Title:

KBTBD2 promotes proliferation and migration of gastric cancer via activating EGFR signaling pathway

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

International journal of biological macromolecules

Application:

IF:

8.2

Species:

Homo sapiens

PMID:

38272402

Title:

Hsa_circ_0086414/transducer of ERBB2 (TOB2) axis-driven lipid elimination and tumor suppression in clear cell renal cell cancer via perilipin 3

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Phytomedicine : international journal of phytotherapy and phytopharmacology

Application:

IF:

6.65

Species:

Homo sapiens

PMID:

37269775

Title:

Sophflarine A, a novel matrine-derived alkaloid from Sophora flavescens with therapeutic potential for non-small cell lung cancer through ROS-mediated pyroptosis

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Molecular carcinogenesis

Application:

IF:

4.6

Species:

Homo sapiens

PMID:

38501385

Title:

The RNAbinding protein sorbin and SH3 domaincontaining 2 are transcriptionally regulated by specificity protein 1 and function as tumor suppressors in bladder

References for [KD Validated] Vimentin Rabbit pAb (A11952)

Product:

[KD Validated] Vimentin Rabbit pAb

Journal:

Open medicine (Warsaw, Poland)

Application:

IF:

Species:

Homo sapiens

PMID:

38025528

Title: