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[KO Validated] PTEN Rabbit mAb (A19104)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KO Validated] PTEN Rabbit mAb (A19104) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

Western blot analysis of lysates from C6 cells, using [KO Validated] PTEN Rabbit mAb (A19104) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

Western blot analysis of lysates from wild type (WT) and PTEN knockout (KO) HeLa cells, using [KO Validated] PTEN Rabbit mAb (A19104) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 60s.

ABclonal:Immunofluorescence - [KO Validated] PTEN Rabbit mAb (A19104)

Confocal imaging of NIH/3T3 cells using [KO Validated] PTEN Rabbit mAb (A19104, dilution 1:100) followed by a further incubation with Cy3 Goat Anti-Rabbit IgG (H+L) (AS007, dilution 1:500) (Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) Ab (AS076, dilution 1:500) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KO Validated] PTEN Rabbit mAb (A19104)

Immunoprecipitation analysis of 300 μg extracts of NIH/3T3 cells using 3 μg PTEN antibody (A19104). Western blot was performed from the immunoprecipitate using PTEN antibody (A19104) at a dilution of 1:1000.

Overview

Product name	[KO Validated] PTEN Rabbit mAb
Catalog No.	A19104
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC2643

Background

This gene was identified as a tumor suppressor that is mutated in a large number of cancers at high frequency. The protein encoded by this gene is a phosphatidylinositol-3, 4, 5-trisphosphate 3-phosphatase. It contains a tensin like domain as well as a catalytic domain similar to that of the dual specificity protein tyrosine phosphatases. Unlike most of the protein tyrosine phosphatases, this protein preferentially dephosphorylates phosphoinositide substrates. It negatively regulates intracellular levels of phosphatidylinositol-3, 4, 5-trisphosphate in cells and functions as a tumor suppressor by negatively regulating AKT/PKB signaling pathway. The use of a non-canonical (CUG) upstream initiation site produces a longer isoform that initiates translation with a leucine, and is thought to be preferentially associated with the mitochondrial inner membrane. This longer isoform may help regulate energy metabolism in the mitochondria. A pseudogene of this gene is found on chromosome 9. Alternative splicing and the use of multiple translation start codons results in multiple transcript variants encoding different isoforms.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-403 of human PTEN (P60484).
Sequence	MTAIIKEIVSRNKRRYQEDGFDLDLTYIYPNIIAMGFPAERLEGVYRNNIDDVVRFLDSKHKNHYKIYNLCAERHYDTAKFNCRVAQYPFEDHNPPQLELIKPFCEDLDQWLSEDDNHVAAIHCKAGKGRTGVMICAYLLHRGKFLKAQEALDFYGEVRTRDKKGVTIPSQRRYVYYYSYLLKNHLDYRPVALLFHKMMFETIPMFSGGTCNPQFVVCQLKVKIYSSNSGPTRREDKFMYFEFPQPLPVCGDIKVEFFHKQNKMLKKDKMFHFWVNTFFIPGPEETSEKVENGSLCDQEIDSICSIERADNDKEYLVLTLTKNDLDKANKDKANRYFSPNFKVKLYFTKTVEEPSNPEASSSTSVTPDVSDNEPDHYRYSDTTDSDPENEPFDEDQHTQITKV
Gene ID
Swiss Prot
Synonyms	BZS; DEC; CWS1; GLM2; MHAM; TEP1; MMAC1; PTEN1; 10q23del; PTENbeta; EN
Calculated MW	47kDa
Observed MW	54kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence Immunoprecipitation
Positive samples	HeLa, NIH/3T3, Mouse testis, Mouse spleen, C6
Cellular location	Cytoplasm, Nucleus, PML body, Secreted.
Customer validation	WB(Homo sapiens, Rattus norvegicus, Mus musculus) IHC(Rattus norvegicus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] PTEN Rabbit mAb images

Western blot - [KO Validated] PTEN Rabbit mAb (A19104)

Immunofluorescence - [KO Validated] PTEN Rabbit mAb (A19104)

Immunoprecipitation - [KO Validated] PTEN Rabbit mAb (A19104)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] PTEN Rabbit mAb (A19104)

Product:

[KO Validated] PTEN Rabbit mAb

Journal:

Drug Dev Res

Application:

IF:

4.36

Species:

Homo sapiens

PMID:

33112006

Title:

Bergenin inhibits bladder cancer progression via activating the PPARγ/PTEN/Akt signal pathway

References for [KO Validated] PTEN Rabbit mAb (A19104)

Product:

[KO Validated] PTEN Rabbit mAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

7.31

Species:

Rattus norvegicus

PMID:

36267811

Title:

Activation of PTEN/P13K/AKT Signaling Pathway by miRNA-124-3p-Loaded Nanoparticles to Regulate Oxidative Stress Attenuates Cardiomyocyte Regulation and Myocardial Injury - PMC

References for [KO Validated] PTEN Rabbit mAb (A19104)

Product:

[KO Validated] PTEN Rabbit mAb

Journal:

Evidence-based complementary and alternative medicine : eCAM

Application:

IF:

2.65

Species:

Rattus norvegicus

PMID:

35899226

Title:

miR-214-3p Protects and Restores the Myocardial Tissue of Rat Myocardial Infarction Model by Targeting PTEN - PMC

References for [KO Validated] PTEN Rabbit mAb (A19104)

Product:

[KO Validated] PTEN Rabbit mAb

Journal:

Experimental cell research

Application:

IHC

IF:

4.14

Species:

Rattus norvegicus

PMID:

36921705

Title:

Silencing EIF3A ameliorates pulmonary arterial hypertension through HDAC1 and PTEN/PI3K/AKT pathway in vitro and in vivo

References for [KO Validated] PTEN Rabbit mAb (A19104)

Product:

[KO Validated] PTEN Rabbit mAb

Journal:

Biochimica et biophysica acta. Molecular cell research

Application:

IF:

5.01

Species:

Homo sapiens

PMID:

37527738

Title:

HTRA3 transcriptionally inhibited by FOXP1 suppresses tumorigenesis of osteosarcoma via the PTEN/PI3K/AKT pathway

References for [KO Validated] PTEN Rabbit mAb (A19104)

Product:

[KO Validated] PTEN Rabbit mAb

Journal:

Molecular therapy. Nucleic acids

Application:

IF:

10.183

Species:

Homo sapiens, Mus musculus

PMID:

35251768

Title:

Overexpression of cytosolic long noncoding RNA cytb protects against pressure-overload-induced heart failure via sponging microRNA-103-3p

References for [KO Validated] PTEN Rabbit mAb (A19104)

Product:

[KO Validated] PTEN Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

Species:

Homo sapiens

PMID:

38664366

Title:

A potential strategy for bladder cancer treatment: inhibiting autophagy to enhance antitumor effects of Nectin-4-MMAE - PMC

References for [KO Validated] PTEN Rabbit mAb (A19104)

Product:

[KO Validated] PTEN Rabbit mAb

Journal:

Journal of biomedical science

Application:

IF:

Species:

Mus musculus

PMID:

39237902

Title:

Tudor-SN exacerbates pathological vascular remodeling by promoting the polyubiquitination of PTEN via NEDD4-1 - PMC

References for [KO Validated] PTEN Rabbit mAb (A19104)

Product:

[KO Validated] PTEN Rabbit mAb

Journal:

Gut microbes

Application:

IF:

12.2

Species:

Mus musculus

PMID:

39410876

Title:

High-fat diet-induced L-saccharopine accumulation inhibits estradiol synthesis and damages oocyte quality by disturbing mitochondrial homeostasis - PMC