RNase Inhibitor, Mammalian specifically inhibits RNases A, B and C. It inhibits RNases by binding noncovalently in a 1:1 ratio with high affinity. It is not effective against RNase 1, RNase T1, S1 Nuclease, RNase H or RNase from Aspergillus. In addition, no inhibition of polymerase activity is observed when RNase Inhibitor is used with Taq DNA Polymerase, AMV or M-MuLV Reverse Transcriptases, or Phage RNA Polymerases (SP6, T7, or T3). The 50 kDa protein inhibits RNases by binding noncovalently in a 1:1 ratio with an association constant greater than 1014.
Product Features
Efficiently inhibit the activity of RNase A, B and C enzymes.
No effect on most PCR and reverse transcriptase activities, not interfere with downstream experiments such as reverse transcription and fluorescence quantification.
Applications
Cell-Free Protein Expression, PCR
Storage
-20°C
Product Information
Product Components
Components
2, 000 U
10, 000 U
RNase Inhibitor, Mammalian (40, 000 U/mL)
50 μL
250 μL
Product Data
Activity test of RNase Inhibitor
Using different amounts of RNA templates, adding 10 ng of RNase A and 40 U RNase inhibitor from different brands for amplification, respectively. The amplification curve showed that the performance of ABclonal RNase inhibitor was similar to that of international brands, and it can effectively inhibit the activity of RNase A without interfering with normal RT-qPCR experiments.
The Cytidine N-Acetyltransferase NAT10 Participates in Peripheral Nerve Injury-Induced Neuropathic Pain by Stabilizing SYT9 Expression in Primary Sensory …
Published:
2023
Journal:
The Journal of neuroscience : the official journal of the Society for Neuroscience
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