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[KD Validated] HDAC1 Rabbit pAb (A0238)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KD Validated] HDAC1 Rabbit pAb (A0238) at 1:2000 dilution incubated at room temperature for 1.5 hours.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020)
Exposure time: 20 s.

Western blot analysis of lysates from wild type (WT) and HDAC1 knockdown (KD) 293T cells, using [KD Validated] HDAC1 Rabbit pAb (A0238) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human colon using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunofluorescence analysis of A549 cells using [KD Validated] HDAC1 Rabbit pAb (A0238). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of L929 cells using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:200. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunoprecipitation analysis of 200 μg extracts of HT-29 cells, using 3 μg [KD Validated] HDAC1 Rabbit pAb (A0238). Western blot was performed from the immunoprecipitate using HDAC1 antibody (A0238) at a dilution of 1:1000.

Review

Overview

Product name	[KD Validated] HDAC1 Rabbit pAb
Catalog No.	A0238
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 393-482 of human HDAC1 (NP_004955.2).
Sequence	SGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKEKDPEEKKEVTEEEKTKEEKPEAKGVKEEVKLA
Gene ID
Swiss Prot
Synonyms	HD1; RPD3; KDAC1; GON-10; RPD3L1; C1
Calculated MW	55kDa
Observed MW	62kDa/

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:100 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T, HeLa, NIH/3T3
Cellular location	Nucleus.
Customer validation	(Human acute leukemia cells, HL60,Hut102, 293T) WB(Homo sapiens, Mus musculus, Sus scrofa, Rattus norvegicus) IP(Rattus norvegicus) ChIP(Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

[KD Validated] HDAC1 Rabbit pAb images

Western blot - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunohistochemistry - [KD Validated] HDAC1 Rabbit pAb (A0238)

Immunofluorescence - [KD Validated] HDAC1 Rabbit pAb (A0238)

Confocal immunofluorescence analysis of L929 cells using [KD Validated] HDAC1 Rabbit pAb (A0238) at dilution of 1:200. Blue: DAPI for nuclear staining.

Immunoprecipitation - [KD Validated] HDAC1 Rabbit pAb (A0238)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for A0238

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Reviews List

Product Protocols

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Neuroscience

Application:

IF:

3.59

Species:

Homo sapiens

PMID:

25779965

Title:

Galangin-induced down-regulation of BACE1 by epigenetic mechanisms in SH-SY5Y cells

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Oncology reports

Application:

IF:

3.906

Species:

Homo sapiens

PMID:

30542715

Title:

Inhibition of miR‑194 suppresses the Wnt/β‑catenin signalling pathway in gastric cancer.

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

The Journal of biological chemistry

Application:

IF:

5.157

Species:

Homo sapiens

PMID:

31694914

Title:

SUMOylation down-regulates rDNA transcription by repressing expression of upstream-binding factor and proto-oncogene c-Myc

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Cell Death Differ

Application:

IF:

15.828

Species:

Mus musculus

PMID:

33024274

Title:

Histone deacetylase 3 aberration inhibits Klotho transcription and promotes renal fibrosis

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Int J Biol Sci

Application:

IF:

6.58

Species:

Mus musculus

PMID:

34803511

Title:

Co-targeting BET bromodomain BRD4 and RAC1 suppresses growth, stemness and tumorigenesis by disrupting the c-MYC-G9a-FTH1axis and downregulating HDAC1 in molecular subtypes of breast cancer

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Mol Cancer

Application:

IF:

27.4

Species:

Homo sapiens

PMID:

34863211

Title:

A novel protein AXIN1-295aa encoded by circAXIN1 activates the Wnt/β-catenin signaling pathway to promote gastric cancer progression

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Journal of cellular physiology

Application:

IF:

6.38

Species:

Homo sapiens

PMID:

30786008

Title:

Regulation of P300 and HDAC1 on endoplasmic reticulum stress in isoniazid‐induced HL‐7702 hepatocyte injury

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

J Virol

Application:

IF:

5.103

Species:

Sus scrofa

PMID:

34232065

Title:

Porcine epidemic diarrhea virus inhibits HDAC1 expression to facilitate its replication via binding of its nucleocapsid protein to host transcription factor Sp1

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Molecular medicine reports

Application:

IF:

3.42

Species:

Homo sapiens

PMID:

31974619

Title:

Chidamide induces necroptosis via regulation of c‑FLIPL expression in Jurkat and HUT‑78 cells

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Nature metabolism

Application:

IF:

19.86

Species:

Homo sapiens

PMID:

36593272

Title:

Lactylome analysis suggests lactylation-dependent mechanisms of metabolic adaptation in hepatocellular carcinoma

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Molecular cancer

Application:

IF:

41.44

Species:

Homo sapiens

PMID:

37884951

Title:

circPSD3 is a promising inhibitor of uPA system to inhibit vascular invasion and metastasis in hepatocellular carcinoma - PMC

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Chemico-biological interactions

Application:

IF:

5.16

Species:

Mus musculus

PMID:

37517432

Title:

Tangeretin and 4-demethyltangeretin prevent damage to mouse hepatocytes from oxidative stress by activating the Nrf2-related antioxidant pathway via an

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Cellular oncology (Dordrecht)

Application:

IF:

7.05

Species:

Homo sapiens

PMID:

37684512

Title:

Synergistic antitumor efficacy of gemcitabine and cisplatin to induce ferroptosis in pancreatic ductal adenocarcinoma via Sp1-SAT1-polyamine metabolism pathway

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

The Journal of biological chemistry

Application:

IF:

Species:

Homo sapiens

PMID:

38072047

Title:

SUMOylation Is Enriched in the Nuclear Matrix and Required for Chromosome Segregation

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Mus musculus

PMID:

38331933

Title:

Glucocorticoids increase adiposity by stimulating Krüppel-like factor 9 expression in macrophages - PMC

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

bioRxiv : the preprint server for biology

Application:

IF:

Species:

Mus musculus

PMID:

38585940

Title:

The human genetic variant rs6190 unveils Foxc1 and Arid5a as novel pro-metabolic targets of the glucocorticoid receptor in muscle.

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Cell Death And Differentiation

Application:

WB, IP, ChIP

IF:

12.067

Species:

Rattus norvegicus

PMID:

28186506

Title:

Epigenetic regulation of HDAC1 SUMOylation as an endogenous neuroprotection against A toxicity in a mouse model of Alzheimer's disease

References for [KD Validated] HDAC1 Rabbit pAb (A0238)

Product:

[KD Validated] HDAC1 Rabbit pAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Mus musculus

PMID:

35915078

Title:

Nucleosome remodeling and deacetylation complex and MBD3 influence mouse embryonic stem cell naïve pluripotency under inhibition of protein kinase C - PMC