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[KO Validated] HDAC1 Rabbit mAb (A19571)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using [KO Validated] HDAC1 Rabbit mAb (A19571) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Western blot analysis of lysates from wild type (WT) and HDAC1 knockout (KO) 293T cells, using [KO Validated] HDAC1 Rabbit mAb (A19571) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

ABclonal:Immunohistochemistry - [KO Validated] HDAC1 Rabbit mAb (A19571)

Immunohistochemistry analysis of paraffin-embedded Mouse lung tissue using [KO Validated] HDAC1 Rabbit mAb (A19571) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse testis tissue using [KO Validated] HDAC1 Rabbit mAb (A19571) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat brain tissue using [KO Validated] HDAC1 Rabbit mAb (A19571) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat colon tissue using [KO Validated] HDAC1 Rabbit mAb (A19571) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Rat testis tissue using [KO Validated] HDAC1 Rabbit mAb (A19571) at a dilution of 1:2000 (40x lens). High pressure antigen retrieval performed with 0.01M Tris-EDTA Buffer (pH 9.0) prior to IHC staining.

ABclonal:Immunofluorescence - [KO Validated] HDAC1 Rabbit mAb (A19571)

Confocal imaging of HeLa cells using [KO Validated] HDAC1 Rabbit mAb (A19571, dilution 1:100)(Red). The cells were counterstained with α-Tubulin Mouse mAb (AC012, dilution 1:400) (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

ABclonal:Immunoprecipitation - [KO Validated] HDAC1 Rabbit mAb (A19571)

Immunoprecipitation analysis of 300ug extracts of 293T cells using 0.5ug HDAC1 antibody (A19571). Western blot was performed from the immunoprecipitate using HDAC1 antibody (A19571) at a dilition of 1:1000.

Overview

Product name	[KO Validated] HDAC1 Rabbit mAb
Catalog No.	A19571
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0050

Background

Histone acetylation and deacetylation, catalyzed by multisubunit complexes, play a key role in the regulation of eukaryotic gene expression. The protein encoded by this gene belongs to the histone deacetylase/acuc/apha family and is a component of the histone deacetylase complex. It also interacts with retinoblastoma tumor-suppressor protein and this complex is a key element in the control of cell proliferation and differentiation. Together with metastasis-associated protein-2, it deacetylates p53 and modulates its effect on cell growth and apoptosis.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 350-450 of human HDAC1 (Q13547).
Sequence	MTNQNTNEYLEKIKQRLFENLRMLPHAPGVQMQAIPEDAIPEESGDEDEDDPDKRISICSSDKRIACEEEFSDSEEEGEGGRKNSSNFKKAKRVKTEDEKE
Gene ID
Swiss Prot
Synonyms	HD1; RPD3; KDAC1; GON-10; RPD3L1; C1
Calculated MW	55kDa
Observed MW	65kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:4000 IHC-P 1:500 - 1:2000 IF/ICC 1:100 - 1:2000 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T, MCF7, NIH/3T3, Mouse thymus, Mouse testis, Rat testis
Cellular location	Nucleus.
Customer validation	IHC(Rattus norvegicus) Co-IP(Homo sapiens) WB(Homo sapiens, Rattus norvegicus, Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

[KO Validated] HDAC1 Rabbit mAb images

Western blot - [KO Validated] HDAC1 Rabbit mAb (A19571)

Immunohistochemistry - [KO Validated] HDAC1 Rabbit mAb (A19571)

Immunofluorescence - [KO Validated] HDAC1 Rabbit mAb (A19571)

Immunoprecipitation - [KO Validated] HDAC1 Rabbit mAb (A19571)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for [KO Validated] HDAC1 Rabbit mAb (A19571)

Product:

[KO Validated] HDAC1 Rabbit mAb

Journal:

Experimental cell research

Application:

IHC

IF:

4.14

Species:

Rattus norvegicus

PMID:

36921705

Title:

Silencing EIF3A ameliorates pulmonary arterial hypertension through HDAC1 and PTEN/PI3K/AKT pathway in vitro and in vivo

References for [KO Validated] HDAC1 Rabbit mAb (A19571)

Product:

[KO Validated] HDAC1 Rabbit mAb

Journal:

The Journal of biological chemistry

Application:

Co-IP

IF:

Species:

Homo sapiens

PMID:

36736423

Title:

Acetylation stabilizes the signaling protein WISP2 by preventing its degradation to suppress the progression of acute myeloid leukemia

References for [KO Validated] HDAC1 Rabbit mAb (A19571)

Product:

[KO Validated] HDAC1 Rabbit mAb

Journal:

Cell death & disease

Application:

IF:

Species:

Homo sapiens

PMID:

37833251

Title:

Critical role of VHL/BICD2/STAT1 axis in crystal-associated kidney disease - PMC

References for [KO Validated] HDAC1 Rabbit mAb (A19571)

Product:

[KO Validated] HDAC1 Rabbit mAb

Journal:

European journal of medicinal chemistry

Application:

IF:

7.08

Species:

Homo sapiens

PMID:

38141287

Title:

Dual-targeting compounds possessing enhanced anticancer activity via microtubule disruption and histone deacetylase inhibition

References for [KO Validated] HDAC1 Rabbit mAb (A19571)

Product:

[KO Validated] HDAC1 Rabbit mAb

Journal:

Journal of enzyme inhibition and medicinal chemistry

Application:

IF:

5.6

Species:

Homo sapiens

PMID:

38465731

Title:

Discovery of novel anaplastic lymphoma kinase (ALK) and histone deacetylase (HDAC) dual inhibitors exhibiting antiproliferative activity against non-small cell lung

References for [KO Validated] HDAC1 Rabbit mAb (A19571)

Product:

[KO Validated] HDAC1 Rabbit mAb

Journal:

Oxidative medicine and cellular longevity

Application:

IF:

6.54

Species:

Rattus norvegicus

PMID:

35571235