| Product name | Rabbit anti-Human IL-1β mAb (CAP) |
|---|---|
| Catalog No. | RM17733 |
| Catagory | Elisa Antibody Kit |
| Application | ELISA, Multiplex, CLIA |
| Description | The protein encoded by this gene is a member of the interleukin 1 cytokine family. This cytokine is produced by activated macrophages as a proprotein, which is proteolytically processed to its active form by caspase 1 (CASP1/ICE). This cytokine is an important mediator of the inflammatory response, and is involved in a variety of cellular activities, including cell proliferation, differentiation, and apoptosis. The induction of cyclooxygenase-2 (PTGS2/COX2) by this cytokine in the central nervous system (CNS) is found to contribute to inflammatory pain hypersensitivity. Similarly, IL-1B has been implicated in human osteoarthritis pathogenesis. Patients with severe Coronavirus Disease 2019 (COVID-19) present elevated levels of pro-inflammatory cytokines such as IL-1B in bronchial alveolar lavage fluid samples. The lung damage induced by the Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is to a large extent, a result of the inflammatory response promoted by cytokines such as IL-1B. This gene and eight other interleukin 1 family genes form a cytokine gene cluster on chromosome 2. |
| Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 117-269 of human IL-1β (NP_000567.1). |
| Purirty | Affinity purification |
| Ig Type | Rabbit IgG |
| Formulation | Supplied as a 0.2μm filtered solution in PBS with 0.05% Proclin300, PH 7.4. |
| CrossReactivity | No cross-reactivity in ELISA recombinant hIL-1A 、hIL-1RA 、hIL-18 、hIL-33、 mIL-1B、 mIL-33 、rIL-1A 、rIL-1B |
| Storage | Store at -20℃. Avoid repeated freeze-thaw cycles. |
Human IL-1β Sandwich Immunoassay
| Recommended Concentration | Sample | ||
|---|---|---|---|
| ELISA | Capture | 0.5-2ug/mL | Rabbit anti-Human IL-1β mAb(Cat. No.RM17733) |
| Detection | 0.05-0.2ug/mL | Rabbit anti-Human IL-1β mAb(Cat. No.RM17734) | |
| Standard | 7.81-500pg/mL | Recombinant Human IL-1β Protein | |
| Multiplex | Capture | / | Rabbit anti-Human IL-1β mAb(Cat. No.RM17733) |
| Detection | / | Rabbit anti-Human IL-1β mAb(Cat. No.RM17734) | |
| Standard | 13.7-10000pg/mL | Recombinant Human IL-1β Protein |
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