Recombinant Human Bcl-2 Protein (RP00062)

Overview

Product name	Recombinant Human Bcl-2 Protein
Catalog No.	RP00062
Description	Recombinant Human Bcl-2 Protein is produced by E. coli expression system. The target protein is expressed with sequence (Ala2-Asp211) of human BCL2 (Accession #NP_000624.2) fused with no tags.
Bio-Activity	Measured by its binding ability in a functional ELISA. Immobilized Human BCL-XL Protein at 2 μg/mL (100 μL/well) can bind BCL2 (Catalog: RP00062) with a linear range of1.95-126.76ng/mL.
Purity	> 90% by SDS-PAGE.
Endotoxin	< 1.0 EU/μg of the protein by LAL method.
Formulation	Lyophilized from a 0.22 μm filtered solution of 20mM HEPES, 50mM KCl, pH 7.5.Contact us for customized product form or formulation.
Species	Human
Calculated MW	23.18 kDa

This protein is an integral outer mitochondrial membrane protein that blocks the apoptotic death of some cells such as lymphocytes. Constitutive expression of BCL2, such as in the case of translocation of BCL2 to Ig heavy chain locus, is thought to be the cause of follicular lymphoma.

Product information

Synonym	BCL2; Bcl-2; PPP1R50; apoptosis regulator Bcl-2; Bcl-2; PPP1R50
Expression Host	E. coli
Tag	No tag
Swiss-Prot
Gene ID

Usage guide

Storage	Store at -20℃.Store the lyophilized protein at -20℃ to -80 ℃ up to 1 year from the date of receipt. After reconstitution, the protein solution is stable at -20℃ for 3 months, at 2-8℃ for up to 1 week.
Reconstitution	Centrifuge the vial before opening. Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water. Avoid vortex or vigorously pipetting the protein. For long term storage, it is recommended to add a carrier protein or stablizer (e.g. 0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose), and aliquot the reconstituted protein solution to minimize free-thaw cycles.

* For research use only. Not for therapeutic or diagnostic purposes.