Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat
Western blot analysis of various lysates using Bcl-2 Mouse mAb (A20777) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Mouse IgG (H+L) (AS003) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.
Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer tissue using Bcl-2 Mouse mAb (A20777) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Human tonsil tissue using Bcl-2 Mouse mAb (A20777) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Mouse kidney tissue using Bcl-2 Mouse mAb (A20777) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunohistochemistry analysis of paraffin-embedded Rat lung tissue using Bcl-2 Mouse mAb (A20777) at a dilution of 1:500 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
Immunofluorescence analysis of HeLa cells using Bcl-2 Mouse mAb (A20777) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008) at 1:500 dilution. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of NIH/3T3 cells using Bcl-2 Mouse mAb (A20777) at a dilution of 1:100 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Mouse IgG (H+L) (AS008) at 1:500 dilution. Blue: DAPI for nuclear staining.
| Product name | Bcl-2 Mouse mAb |
|---|---|
| Catalog No. | A20777 |
| Host species | Mouse |
| Purification method | Affinity purification |
| Isotype | IgG1, Kappa |
| CloneNo. | AMC0441 |
| Immunogen | Recombinant protein of mouse Bcl-2. |
|---|---|
| Sequence | Email for sequence |
| Gene ID | |
| Swiss Prot | |
| Synonyms | Bcl-2; PPP1R50 |
| Calculated MW | 26kDa |
| Observed MW | 26kDa |
| Reactivity | Human, Mouse, Rat |
|---|---|
| Tested applications | WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition |
| Recommended dilution |
|
| Storage buffer | Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3. |
| Key application | Western blotting Immunohistochemistry Immunofluorescence |
| Positive samples | Raji, Jurkat, 293T, NIH/3T3 |
| Cellular location | Endoplasmic reticulum membrane, Mitochondrion outer membrane, Nucleus membrane, Single-pass membrane protein. |
| Customer validation | IF(Homo sapiens) WB(Homo sapiens, Rattus norvegicus , Mus musculus, Homo sapiens) |
To download a Certificate of Compliance, please enter your Lot number below:
Lot number
* For research use only. Not for therapeutic or diagnostic purposes.
Publishing research using A20777? Please let us know so that we can cite the reference in this datasheet.
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