제품 > 항체 > 다중클론항체(pAb)

c-Jun Rabbit pAb (A0246)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using c-Jun Rabbit pAb (A0246) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - c-Jun Rabbit pAb (A0246)

Immunofluorescence analysis of NIH/3T3 cells using c-Jun Rabbit pAb (A0246) at dilution of 1:100. Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	c-Jun Rabbit pAb
Catalog No.	A0246
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 90-230 of c-Jun (NP_002219.1).
Sequence	TTPTPTQFLCPKNVTDEQEGFAEGFVRALAELHSQNTLPSVTSAAQPVNGAGMVAPAVASVAGGSGSGGFSASLHSEPPVYANLSNFNPGALSSGGGAPSYGAAGLAFPAQPQQQQQPPHHLPQQMPVQHPRLQALKEEPQ
Gene ID
Swiss Prot
Synonyms	AP1; p39; AP-1; cJUN; c-Jun
Calculated MW	36kDa
Observed MW	46kDa/42kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	HeLa, HT-1080, NIH/3T3
Cellular location	Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus, Rattus norvegicus, Gallus gallus, Sus scrofa) IHC(Rattus norvegicus) ChIP(Oryza sativa)

Documents

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Research Area

c-Jun Rabbit pAb images

Western blot - c-Jun Rabbit pAb (A0246)

Immunofluorescence - c-Jun Rabbit pAb (A0246)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Science Signaling

Application:

IF:

8.192

Species:

Homo sapiens

PMID:

27703031

Title:

Quantitative phosphoproteomic analysis identifies the critical role of JNK1 in neuroinflammation induced by Japanese encephalitis virus

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

Application:

IF:

5.293

Species:

Homo sapiens

PMID:

28680855

Title:

p21-Activated Kinase 4 Signaling Promotes Japanese Encephalitis Virus-Mediated Inflammation in Astrocytes

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Gastroenterology

Application:

IF:

22.682

Species:

Homo sapiens

PMID:

25449213

Title:

The long intergenic noncoding RNA UFC1, a target of MicroRNA 34a, interacts with the mRNA stabilizing protein HuR to increase levels of β-catenin in HCC cells

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

BMC medicine

Application:

IF:

8.775

Species:

Mus musculus

PMID:

30782145

Title:

Cathelicidin-related antimicrobial peptide protects against myocardial ischemia/reperfusion injury.

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Oncotarget

Application:

IF:

3.765

Species:

Homo sapiens

PMID:

26943030

Title:

UBE2T promotes nasopharyngeal carcinoma cell proliferation, invasion, and metastasis by activating the AKT/GSK3β/β-catenin pathway.

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Biochem Pharmacol

Application:

IF:

5.858

Species:

Homo sapiens

PMID:

33080187

Title:

Glutathione S-transferases P1-mediated interleukin-6 in tumor-associated macrophages augments drug-resistance in MCF-7 breast cancer

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Theranostics

Application:

IF:

11.556

Species:

Homo sapiens

PMID:

33456583

Title:

Cytoplasmic SHMT2 drives the progression and metastasis of colorectal cancer by inhibiting β-catenin degradation

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Rattus norvegicus

PMID:

35397390

Title:

Network pharmacology-based strategy to investigate pharmacological mechanisms of Andrographolide for treatment of vascular cognitive impairment

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

36292923

Title:

Diclofenac Sensitizes Signet Ring Cell Gastric Carcinoma Cells to Cisplatin by Activating Autophagy and Inhibition of Survival Signal Pathways - PMC

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Journal of agricultural and food chemistry

Application:

IF:

5.89

Species:

Gallus gallus

PMID:

35128924

Title:

Glycyrrhizic Acid against Mycoplasma gallisepticum-Induced Inflammation and Apoptosis Through Suppressing the MAPK Pathway in Chickens

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Mus musculus

PMID:

36467049

Title:

Exploring the potential mechanism of Fritiliariae Irrhosae Bulbus on ischemic stroke based on network pharmacology and experimental validation - PMC

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Human gene therapy

Application:

IF:

4.79

Species:

Homo sapiens

PMID:

36373826

Title:

HIV Tat-conjugated Histone H3 peptides induce tumor cell death via cellular stress responses

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Endocrinology

Application:

IF:

5.05

Species:

Rattus norvegicus

PMID:

36928142

Title:

Cadmium-induced Sertoli cell injury through p38-MAPK and related signaling proteins–a study by RNA sequencing

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Mus musculus

PMID:

37279643

Title:

Identification and verification of hub genes associated with ferroptosis in ischemia and reperfusion injury during renal transplantation

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Journal of biochemical and molecular toxicology

Application:

IF:

3.56

Species:

Sus scrofa

PMID:

37706587

Title:

Oleanolic acid attenuates hydrogen peroxideinduced apoptosis of IPECJ2 cells through suppressing cJun and MAPK pathway

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

American journal of translational research

Application:

IHC

IF:

3.94

Species:

Rattus norvegicus

PMID:

37560235

Title:

Down-regulation of Jun induces senescence through destabilizing chromatin in osteoarthritis chondrocytes - PMC

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

American journal of translational research

Application:

IHC

IF:

3.94

Species:

Rattus norvegicus

PMID:

37560208

Title:

The molecular link between obstructive sleep apnea and osteoarthritis: based on bioinformatics analysis and experimental validation - PMC

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Frontiers in Immunology

Application:

IF:

8.786

Species:

Mus musculus

PMID:

29910805

Title:

IP-10 Promotes Blood-Brain Barrier Damage by Inducing Tumor Necrosis Factor Alpha Production in Japanese Encephalitis

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Insect biochemistry and molecular biology

Application:

ChIP

IF:

4.42

Species:

Oryza sativa

PMID:

36535580

Title:

c-Jun regulates flotillin 2 transcription to benefit viral accumulation in insect vectors

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

Molecules (Basel, Switzerland)

Application:

IF:

4.92

Species:

Mus musculus

PMID:

36985786

Title:

Ethanol Extract of Rosa laevigata Michx. Fruit Inhibits Inflammatory Responses through NF-B/MAPK Signaling Pathways via AMPK Activation in RAW 264.7 Macrophages - PMC

References for c-Jun Rabbit pAb (A0246)

Product:

c-Jun Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

38026210