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Phospho-c-Jun-S73 Rabbit pAb (AP0047)

Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - Phospho-c-Jun-S73 Rabbit pAb (AP0047)

Western blot analysis of lysates from C6 cells, using Phospho-c-Jun-S73 Rabbit pAb (AP0047) at 1:400 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-c-Jun-S73 Rabbit pAb (AP0047)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-c-Jun-S73 Rabbit pAb (AP0047) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Overview

Product namePhospho-c-Jun-S73 Rabbit pAb
Catalog No.AP0047
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.
ImmunogenA synthetic phosphorylated peptide around S73 of human c-Jun (NP_002219.1).
SequenceLASPE
Gene ID
Swiss Prot
SynonymsAP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-S73
Calculated MW36kDa
Observed MW45-48kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:100 - 1:500
  • IHC-P 1:50 - 1:200
  • ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    
Positive samplesC6 treated by UV
Cellular locationNucleus.

Documents

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ABclonal:Western blot - Phospho-c-Jun-S73 Rabbit pAb (AP0047)}

Western blot - Phospho-c-Jun-S73 Rabbit pAb (AP0047)

Western blot analysis of lysates from C6 cells, using Phospho-c-Jun-S73 Rabbit pAb (AP0047) at 1:400 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.
ABclonal:Immunohistochemistry - Phospho-c-Jun-S73 Rabbit pAb (AP0047)}

Immunohistochemistry - Phospho-c-Jun-S73 Rabbit pAb (AP0047)

Immunohistochemistry analysis of paraffin-embedded Human breast cancer using Phospho-c-Jun-S73 Rabbit pAb (AP0047) at dilution of 1:20 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

* For research use only. Not for therapeutic or diagnostic purposes.

항체 (10)

Secondary Antibodies (26)