제품 > 항체 > 인산화 특이적 항체

Phospho-c-Jun-T91 Rabbit pAb (AP1216)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Phospho-c-Jun-T91 Rabbit pAb (AP1216) at 1:1000 dilution. Both 293T cells and NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes after serum-starvation overnight. Both 293T cells and NIH/3T3 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

ABclonal:Immunohistochemistry - Phospho-c-Jun-T91 Rabbit pAb (AP1216)

Immunohistochemistry analysis of paraffin-embedded Rat lung using Phospho-c-Jun-T91 Rabbit pAb (AP1216) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human liver cancer using Phospho-c-Jun-T91 Rabbit pAb (AP1216) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Mouse kidney using Phospho-c-Jun-T91 Rabbit pAb (AP1216) at dilution of 1:50 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - Phospho-c-Jun-T91 Rabbit pAb (AP1216)

Immunofluorescence analysis of U2OS cells using Phospho-c-Jun-T91 Rabbit pAb (AP1216) at dilution of 1:50 (40x lens). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-c-Jun-T91 Rabbit pAb
Catalog No.	AP1216
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T91 of human JUN (NP_002219.1).
Sequence	TTTPT
Gene ID
Swiss Prot
Synonyms	AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-T91
Calculated MW	36kDa
Observed MW	36kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence
Positive samples	293T treated by Anisomycin, 293T treated by UV, NIH/3T3 treated by Anisomycin, NIH/3T3 treated by UV
Cellular location	Nucleus.