제품 > 항체 > 다중클론항체(pAb)

c-Jun Rabbit pAb (A11378)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from Rat kidney, using c-Jun Rabbit pAb (A11378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Western blot analysis of lysates from MCF7 cells, using c-Jun Rabbit pAb (A11378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 10s.

Western blot analysis of various lysates using c-Jun Rabbit pAb (A11378) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 30s.

Overview

Product name	c-Jun Rabbit pAb
Catalog No.	A11378
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-70 of human c-Jun (NP_002219.1).
Sequence	MTAKMETTFYDDALNASFLPSESGPYGYSNPKILKQSMTLNLADPVGSLKPHLRAKNSDLLTSPDVGLLK
Gene ID
Swiss Prot
Synonyms	AP1; p39; AP-1; cJUN; c-Jun
Calculated MW	36kDa
Observed MW	43kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	293T, NIH/3T3, MCF7, Mouse lung, Mouse heart, Rat kidney
Cellular location	Nucleus.
Customer validation	IHC(Mus musculus, Homo sapiens, Cervus nippon) WB(Mus musculus, Homo sapiens) RNA-Seq(Homo sapiens) WB(Homo sapiens)

Documents

Certificate of Compliance

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Research Area

c-Jun Rabbit pAb images

Western blot - c-Jun Rabbit pAb (A11378)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for c-Jun Rabbit pAb (A11378)

Product:

c-Jun Rabbit pAb

Journal:

American Journal of Translational Research

Application:

IHC

IF:

4.06

Species:

Mus musculus

PMID:

32269724

Title:

Exploring cisplatin resistance in ovarian cancer through integrated bioinformatics approach and overcoming chemoresistance with sanguinarine

References for c-Jun Rabbit pAb (A11378)

Product:

c-Jun Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.81

Species:

Mus musculus

PMID:

34987399

Title:

Molecular Mechanism of the Regulatory Effect of Schisandrol A on the Immune Function of Mice Based on a Transcription Factor Regulatory Network

References for c-Jun Rabbit pAb (A11378)

Product:

c-Jun Rabbit pAb

Journal:

Annals of clinical and laboratory science

Application:

IHC

IF:

1.18

Species:

Homo sapiens

PMID:

32581025

Title:

Evi-1 influence on clinical progress of colorectal cancer patients

References for c-Jun Rabbit pAb (A11378)

Product:

c-Jun Rabbit pAb

Journal:

Cell regeneration (London, England)

Application:

IHC

IF:

Species:

Cervus nippon

PMID:

36542206

Title:

Single-cell transcriptome reveals core cell populations and androgen-RXFP2 axis involved in deer antler full regeneration

References for c-Jun Rabbit pAb (A11378)

Product:

c-Jun Rabbit pAb

Journal:

Clinical epigenetics

Application:

IF:

7.25

Species:

Mus musculus

PMID:

37951958

Title:

Histone deacetylase 3 deletion in alveolar type 2 epithelial cells prevents bleomycin-induced pulmonary fibrosis - PMC

References for c-Jun Rabbit pAb (A11378)

Product:

c-Jun Rabbit pAb

Journal:

International journal of molecular sciences

Application:

IF:

6.2

Species:

Homo sapiens

PMID:

37894738

Title:

Notopterol Suppresses IL-17-Induced Proliferation and Invasion of A549 Lung Adenocarcinoma Cells via Modulation of STAT3, NF-B, and AP-1 Activation - PMC

References for c-Jun Rabbit pAb (A11378)

Product:

c-Jun Rabbit pAb

Journal:

MedComm

Application:

IF:

Species:

Homo sapiens

PMID:

37063610

Title: