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Phospho-c-Jun-T93 Rabbit pAb (AP1218)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse

Western blot analysis of various lysates using Phospho-c-Jun-T93 Rabbit pAb (AP1218) at 1:500 dilution. HeLa cells were treated by PMA/TPA (200 nM) at 37℃ for 15 minutes after serum-starvation overnight. NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 180s.

Overview

Product name	Phospho-c-Jun-T93 Rabbit pAb
Catalog No.	AP1218
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around T93 of human JUN (NP_002219.1).
Sequence	TPTPT
Gene ID
Swiss Prot
Synonyms	AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-T93
Calculated MW	36kDa
Observed MW	48kDa

Applications

Reactivity	Human, Mouse
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:100 - 1:500
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.01% thimerosal, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	HeLa treated by PMA, NIH/3T3 treated by Anisomycin
Cellular location	Nucleus.