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Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from NIH/3T3 cells, using Phospho-c-Jun-S63 Rabbit mAb (AP0105) at 1:1000 dilution. NIH/3T3 cells were treated by Anisomycin (25 μg/mL) at 37℃ for 30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunofluorescence - Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Immunofluorescence analysis of C6 cells using Phospho-c-Jun-S63 Rabbit mAb (AP0105). Secondary antibody: Cy3-conjugated Goat anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product name	Phospho-c-Jun-S63 Rabbit mAb
Catalog No.	AP0105
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0051

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S63 of human c-Jun (P05412).
Sequence	LTSPDV
Gene ID
Swiss Prot
Synonyms	AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-S63
Calculated MW	36kDa
Observed MW	48kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	NIH/3T3 treated by Anisomycin
Cellular location	Nucleus.
Customer validation	WB(Rattus norvegicus, Mus musculus, Homo sapiens) IP(mus musculus) IF(Mus musculus) WB (Mus musculus)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-c-Jun-S63 Rabbit mAb images

Western blot - Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Immunofluorescence - Phospho-c-Jun-S63 Rabbit mAb (AP0105)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Product:

Phospho-c-Jun-S63 Rabbit mAb

Journal:

Endocrinology

Application:

IF:

5.05

Species:

Rattus norvegicus

PMID:

36928142

Title:

Cadmium-induced Sertoli cell injury through p38-MAPK and related signaling proteins–a study by RNA sequencing

References for Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Product:

Phospho-c-Jun-S63 Rabbit mAb

Journal:

Virology

Application:

IF:

3.51

Species:

Mus musculus

PMID:

36996689

Title:

Zika virus replication on endothelial cells and invasion into the central nervous system by inhibiting interferon β translation

References for Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Product:

Phospho-c-Jun-S63 Rabbit mAb

Journal:

Molecular neurobiology

Application:

IF:

5.68

Species:

mus musculus

PMID:

37955806

Title:

DLK-MAPK Signaling Coupled with DNA Damage Promotes Intrinsic Neurotoxicity Associated with Non-Mutated Tau

References for Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Product:

Phospho-c-Jun-S63 Rabbit mAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

38113968

Title:

Levofloxacin alleviates blood-brain barrier disruption following cerebral ischemia and reperfusion via directly inhibiting A-FABP

References for Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Product:

Phospho-c-Jun-S63 Rabbit mAb

Journal:

Archives of biochemistry and biophysics

Application:

IF:

4.11

Species:

Homo sapiens

PMID:

38367794

Title:

Dihydrocelastrol induces cell death and suppresses angiogenesis through BCR/AP-1/junb signalling in diffuse large B cell lymphoma

References for Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Product:

Phospho-c-Jun-S63 Rabbit mAb

Journal:

Research square

Application:

WB, IF

IF:

Species:

Mus musculus

PMID:

36945524

Title:

Modeling Alzheimers disease in primary neurons reveals DNA damage response coupled with MAPK-DLK signaling in wild-type tau-induced neurodegeneration - PMC

References for Phospho-c-Jun-S63 Rabbit mAb (AP0105)

Product:

Phospho-c-Jun-S63 Rabbit mAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

37035004