제품 > 항체 > 인산화 특이적 항체

Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Rat

Western blot analysis of lysates from C6 cells, using Phospho-c-Jun-S63 Rabbit pAb (AP0048) at 1:1000 dilution. C6 cells were treated by UV at room temperature for 15-30 minutes.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

Review

Overview

Product name	Phospho-c-Jun-S63 Rabbit pAb
Catalog No.	AP0048
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene is the putative transforming gene of avian sarcoma virus 17. It encodes a protein which is highly similar to the viral protein, and which interacts directly with specific target DNA sequences to regulate gene expression. This gene is intronless and is mapped to 1p32-p31, a chromosomal region involved in both translocations and deletions in human malignancies.

Immunogen information

Immunogen	A synthetic phosphorylated peptide around S63 of human c-Jun (NP_002219.1).
Sequence	LTSPD
Gene ID
Swiss Prot
Synonyms	AP1; p39; AP-1; cJUN; c-Jun; Phospho-c-Jun-S63
Calculated MW	36kDa
Observed MW	48kDa

Applications

Reactivity	Human, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting
Positive samples	C6 treated by UV
Cellular location	Nucleus.
Customer validation	(Mouse embryonal fibroblast cell, 293T) WB(Homo sapiens, Mus musculus, Sus scrofa, Gallus gallus, Rattus norvegicus, Oryza sativa)

Documents

Certificate of Compliance

To download a Certificate of Compliance, please enter your Lot number below:

Lot number

Research Area

Phospho-c-Jun-S63 Rabbit pAb images

Western blot - Phospho-c-Jun-S63 Rabbit pAb (AP0048)

* For research use only. Not for therapeutic or diagnostic purposes.

Submit an review for AP0048

Please submit reviews to your technical sales specialist directly. Alternatively, email us at info@abclonal.co.kr

Reviews List

Product Protocols

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

Science Signaling

Application:

IF:

8.192

Species:

Homo sapiens

PMID:

27703031

Title:

Quantitative phosphoproteomic analysis identifies the critical role of JNK1 in neuroinflammation induced by Japanese encephalitis virus

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY

Application:

IF:

5.293

Species:

Homo sapiens

PMID:

28680855

Title:

p21-Activated Kinase 4 Signaling Promotes Japanese Encephalitis Virus-Mediated Inflammation in Astrocytes

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

Journal of experimental & clinical cancer research

Application:

IF:

11.161

Species:

Mus musculus

PMID:

30841931

Title:

Protein kinase Ds promote tumor angiogenesis through mast cell recruitment and expression of angiogenic factors in prostate cancer microenvironment.

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

Veterinary Microbiology

Application:

IF:

3.293

Species:

Sus scrofa

PMID:

31648727

Title:

Japanese Encephalitis Virus infection induces inflammation of swine testis through RIG-I-NF-ĸB signaling pathway

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

Frontiers in immunology

Application:

IF:

8.78

Species:

Gallus gallus

PMID:

35833119

Title:

Lnc90386 Sponges miR-33-5p to Mediate Mycoplasma gallisepticum-Induced Inflammation and Apoptosis in Chickens via the JNK Pathway - PMC

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

Molecular medicine reports

Application:

IF:

3.42

Species:

Homo sapiens

PMID:

29207192

Title:

Resveratrol attenuates hydrogen peroxide‑induced apoptosis, reactive oxygen species generation, and PSGL‑1 and VWF activation in human umbilical vein endothelial cells, potentially via MAPK signalling pathways

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Rattus norvegicus

PMID:

35397390

Title:

Network pharmacology-based strategy to investigate pharmacological mechanisms of Andrographolide for treatment of vascular cognitive impairment

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

Frontiers in pharmacology

Application:

IF:

5.98

Species:

Mus musculus

PMID:

36467049

Title:

Exploring the potential mechanism of Fritiliariae Irrhosae Bulbus on ischemic stroke based on network pharmacology and experimental validation - PMC

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

Drug design, development and therapy

Application:

IF:

4.31

Species:

Homo sapiens

PMID:

36969705

Title:

Icariin Induces Triple-Negative Breast Cancer Cell Apoptosis and Suppresses Invasion by Inhibiting the JNK/c-Jun Signaling Pathway - PMC

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

International immunopharmacology

Application:

IF:

5.71

Species:

Homo sapiens

PMID:

37543013

Title:

LncRNA THRIL, transcriptionally activated by AP-1 and stabilized by METTL14-mediated m6A modification, accelerates LPS-evoked acute injury in alveolar epithelial

References for Phospho-c-Jun-S63 Rabbit pAb (AP0048)

Product:

Phospho-c-Jun-S63 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

9.685

Species:

Homo sapiens

PMID:

35361778