제품 > 항체 > 단일클론항체(mAb)

Bad Rabbit mAb (A19595)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of various lysates using Bad Rabbit mAb (A19595) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 3min.

ABclonal:Immunohistochemistry - Bad Rabbit mAb (A19595)

Immunohistochemistry analysis of paraffin-embedded Human placenta using Bad Rabbit mAb (A19595) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Overview

Product name	Bad Rabbit mAb
Catalog No.	A19595
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG
CloneNo.	ARC0074

Background

The protein encoded by this gene is a member of the BCL-2 family. BCL-2 family members are known to be regulators of programmed cell death. This protein positively regulates cell apoptosis by forming heterodimers with BCL-xL (B-cell lymphoma-extra large) and BCL-2, and reversing their death repressor activity. Proapoptotic activity of this protein is regulated through its phosphorylation. Protein kinases AKT and MAP kinase, as well as protein phosphatase calcineurin were found to be involved in the regulation of this protein. Alternative splicing of this gene results in two transcript variants which encode the same isoform.

Immunogen information

Immunogen	A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Bad (Q92934).
Sequence	MFQIPEFEPSEQEDSSSAERGLGPSPAGDGPSGSGKHHRQAPGLLWDASHQQEQPTSSSHHGGAGAVEIRSRHSSYPAGTEDDEGMGEEPSPFRGRSRSA
Gene ID
Swiss Prot
Synonyms	BBC2; BCL2L8; Bad
Calculated MW	18kDa
Observed MW	20kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:2000 IHC-P 1:200 - 1:800 ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 0.05% BSA, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry
Positive samples	HeLa, A549, Mouse brain, Mouse thymus, Mouse lung, Rat brain
Cellular location	Cytoplasm, Mitochondrion outer membrane.
Customer validation	WB(Mus musculus, Homo sapiens, us scrofa, Rattus norvegicus)

Documents

Certificate of Compliance

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Lot number

Research Area

Bad Rabbit mAb images

Western blot - Bad Rabbit mAb (A19595)

Immunohistochemistry - Bad Rabbit mAb (A19595)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

Int Immunopharmacol

Application:

IF:

4.93

Species:

Mus musculus

PMID:

34358859

Title:

Methyl eugenol attenuates liver ischemia reperfusion injury via activating PI3KAkt signaling

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

Front Oncol

Application:

IF:

6.24

Species:

Mus musculus

PMID:

34900710

Title:

Carnosic Acid Suppresses the Development of Oral Squamous Cell Carcinoma via Mitochondrial-Mediated Apoptosis

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

iScience

Application:

IF:

6.1

Species:

Homo sapiens

PMID:

35789855

Title:

A small-molecule Skp1 inhibitor elicits cell death by p53-dependent mechanism - PMC

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

European journal of pharmacology

Application:

IF:

5.19

Species:

Mus musculus

PMID:

35339477

Title:

CircRNA CDR1as promotes cardiomyocyte apoptosis through activating hippo signaling pathway in diabetic cardiomyopathy

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

Toxics

Application:

IF:

4.47

Species:

us scrofa

PMID:

37999575

Title:

N-Acetyl-L-Cysteine Ameliorates BPAF-Induced Porcine Sertoli Cell Apoptosis and Cell Cycle Arrest via Inhibiting the ROS Level - PMC

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

Cell death discovery

Application:

IF:

7.1

Species:

Homo sapiens

PMID:

38086844

Title:

Cepharanthine, a regulator of keap1-Nrf2, inhibits gastric cancer growth through oxidative stress and energy metabolism pathway - PMC

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

Biochimica et biophysica acta. Molecular cell research

Application:

IF:

5.01

Species:

Mus musculus

PMID:

38182060

Title:

GDF11 mitigates high glucose-induced cardiomyocytes apoptosis by inhibiting the ALKBH5-FOXO3-CDR1as/Hippo signaling pathway

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

Scientific reports

Application:

IF:

4.6

Species:

Homo sapiens,Mus musculus

PMID:

38480798

Title:

Nortriptyline hydrochloride, a potential candidate for drug repurposing, inhibits gastric cancer by inducing oxidative stress by triggering the Keap1-Nrf2

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

Journal of translational medicine

Application:

IF:

8.44

Species:

Mus musculus

PMID:

37422640

Title:

Targeting macrophagic PIM-1 alleviates osteoarthritis by inhibiting NLRP3 inflammasome activation via suppressing mitochondrial ROS/Cl- efflux signaling pathway - PMC

References for Bad Rabbit mAb (A19595)

Product:

Bad Rabbit mAb

Journal:

The Journal of surgical research

Application:

IF:

2.2

Species:

Rattus norvegicus

PMID:

30502252