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SOCS1 Rabbit pAb (A7754)

Publications (8) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:Human, Mouse, Rat

ABclonal:Western blot - SOCS1 Rabbit pAb (A7754)

Western blot analysis of various lysates, using SOCS1 Rabbit pAb (A7754) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - SOCS1 Rabbit pAb (A7754)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SOCS1 Rabbit pAb (A7754) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SOCS1 Rabbit pAb (A7754)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using SOCS1 Rabbit pAb (A7754) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SOCS1 Rabbit pAb (A7754)

Immunohistochemistry analysis of paraffin-embedded Rat brain using SOCS1 Rabbit pAb (A7754) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunohistochemistry - SOCS1 Rabbit pAb (A7754)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using SOCS1 Rabbit pAb (A7754) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.

ABclonal:Immunofluorescence - SOCS1 Rabbit pAb (A7754)

Immunofluorescence analysis of PC-12 cells using SOCS1 Rabbit pAb (A7754) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Overview

Product nameSOCS1 Rabbit pAb
Catalog No.A7754
Host speciesRabbit
Purification methodAffinity purification
IsotypeIgG
This gene encodes a member of the STAT-induced STAT inhibitor (SSI), also known as suppressor of cytokine signaling (SOCS), family. SSI family members are cytokine-inducible negative regulators of cytokine signaling. The expression of this gene can be induced by a subset of cytokines, including IL2, IL3 erythropoietin (EPO), CSF2/GM-CSF, and interferon (IFN)-gamma. The protein encoded by this gene functions downstream of cytokine receptors, and takes part in a negative feedback loop to attenuate cytokine signaling. Knockout studies in mice suggested the role of this gene as a modulator of IFN-gamma action, which is required for normal postnatal growth and survival.
ImmunogenRecombinant fusion protein containing a sequence corresponding to amino acids 1-100 of human SOCS1 (NP_003736.1).
SequenceMVAHNQVAADNAVSTAAEPRRRPEPSSSSSSSPAAPARPRPCPAVPAPAPGDTHFRTFRSHADYRRITRASALLDACGFYWGPLSVHGAHERLRAEPVGT
Gene ID
Swiss Prot
SynonymsJAB; CIS1; SSI1; TIP3; CISH1; SSI-1; TIP-3; AISIMD; SOCS-1; SOCS1
Calculated MW24kDa
Observed MW23kDa
ReactivityHuman, Mouse, Rat
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • WB 1:500 - 1:1000
  • IHC-P 1:50 - 1:200
  • IF/ICC 1:50 - 1:200
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.05% proclin300, 50% glycerol, pH7.3.
Key applicationWestern blotting    Immunohistochemistry    Immunofluorescence    
Positive samplesK-562, Mouse lung, Mouse spleen
Cellular locationCytoplasmic vesicle, Nucleus
Customer validation

WB(Mus musculus, Rattus norvegicus, Homo sapiens, Arabidopsis thaliana)

Co-IP(Arabidopsis thaliana)

Documents

Certificate of Compliance

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ABclonal:Western blot - SOCS1 Rabbit pAb (A7754)}

Western blot - SOCS1 Rabbit pAb (A7754)

Western blot analysis of various lysates, using SOCS1 Rabbit pAb (A7754) at 1:500 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.
ABclonal:Immunohistochemistry - SOCS1 Rabbit pAb (A7754)}

Immunohistochemistry - SOCS1 Rabbit pAb (A7754)

Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using SOCS1 Rabbit pAb (A7754) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SOCS1 Rabbit pAb (A7754)}

Immunohistochemistry - SOCS1 Rabbit pAb (A7754)

Immunohistochemistry analysis of paraffin-embedded Mouse spleen using SOCS1 Rabbit pAb (A7754) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SOCS1 Rabbit pAb (A7754)}

Immunohistochemistry - SOCS1 Rabbit pAb (A7754)

Immunohistochemistry analysis of paraffin-embedded Rat brain using SOCS1 Rabbit pAb (A7754) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunohistochemistry - SOCS1 Rabbit pAb (A7754)}

Immunohistochemistry - SOCS1 Rabbit pAb (A7754)

Immunohistochemistry analysis of paraffin-embedded Rat spleen using SOCS1 Rabbit pAb (A7754) at dilution of 1:200 (40x lens). Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IHC staining protocol.
ABclonal:Immunofluorescence - SOCS1 Rabbit pAb (A7754)}

Immunofluorescence - SOCS1 Rabbit pAb (A7754)

Immunofluorescence analysis of PC-12 cells using SOCS1 Rabbit pAb (A7754) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

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