제품 > 항체 > 다중클론항체(pAb)

GLP1R Rabbit pAb (A8547)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Mouse, Rat

Western blot analysis of extracts of various cell lines, using GLP1R antibody (A8547) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunofluorescence - GLP1R Rabbit pAb (A8547)

Immunofluorescence analysis of L929 cells using GLP1R antibody (A8547) at dilution of 1:100. Blue: DAPI for nuclear staining.

Overview

Product name	GLP1R Rabbit pAb
Catalog No.	A8547
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a 7-transmembrane protein that functions as a receptor for glucagon-like peptide 1 (GLP-1) hormone, which stimulates glucose-induced insulin secretion. This receptor, which functions at the cell surface, becomes internalized in response to GLP-1 and GLP-1 analogs, and it plays an important role in the signaling cascades leading to insulin secretion. It also displays neuroprotective effects in animal models. Polymorphisms in this gene are associated with diabetes. The protein is an important drug target for the treatment of type 2 diabetes and stroke. Alternative splicing of this gene results in multiple transcript variants.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 1-140 of human GLP1R (NP_002053.3).
Sequence	MAGAPGPLRLALLLLGMVGRAGPRPQGATVSLWETVQKWREYRRQCQRSLTEDPPPATDLFCNRTFDEYACWPDGEPGSFVNVSCPWYLPWASSVPQGHVYRFCTAEGLWLQKDNSSLPWRDLSECEESKRGERSSPEEQ
Gene ID
Swiss Prot
Synonyms	GLP-1; GLP-1R; GLP-1-R
Calculated MW	53kDa
Observed MW	53kDa

Applications

Reactivity	Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:2000 IF/ICC 1:50 - 1:200
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunofluorescence
Positive samples	Mouse lung, Rat stomach, Rat lung
Cellular location	Cell membrane, Multi-pass membrane protein
Customer validation	IHC(Mus musculus) WB(Mus musculus, Homo sapiens, Rattus norvegicus)

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GLP1R Rabbit pAb images

Western blot - GLP1R Rabbit pAb (A8547)

Immunofluorescence - GLP1R Rabbit pAb (A8547)

Immunofluorescence analysis of L929 cells using GLP1R antibody (A8547) at dilution of 1:100. Blue: DAPI for nuclear staining.

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for GLP1R Rabbit pAb (A8547)

Product:

GLP1R Rabbit pAb

Journal:

DNA and cell biology

Application:

IHC

IF:

3.311

Species:

Mus musculus

PMID:

30403540

Title:

Liraglutide Promotes the Osteogenic Differentiation in MC3T3-E1 Cells Via Regulating the Expression of Smad2/3 Through PI3K/Akt and Wnt/β-Catenin Pathways.

References for GLP1R Rabbit pAb (A8547)

Product:

GLP1R Rabbit pAb

Journal:

Eur J Pharmacol

Application:

IF:

4.432

Species:

Mus musculus

PMID:

32621911

Title:

Exogenous supplement of glucagon like peptide-1 protects the heart against aortic banding induced myocardial fibrosis and dysfunction through inhibiting mTOR/p70S6K signaling and promoting autophagy

References for GLP1R Rabbit pAb (A8547)

Product:

GLP1R Rabbit pAb

Journal:

Mol Cell Endocrinol

Application:

IF:

4.102

Species:

Mus musculus

PMID:

32877753

Title:

Glucagon-like peptide 1 treatment reverses vascular remodelling by downregulating matrix metalloproteinase 1 expression through inhibition of the ERK1/2/NF-κB …

References for GLP1R Rabbit pAb (A8547)

Product:

GLP1R Rabbit pAb

Journal:

Neurobiol Learn Mem

Application:

IF:

2.877

Species:

Mus musculus

PMID:

34015440

Title:

GLP-1R activation ameliorated novel-object recognition memory dysfunction via regulating hippocampal AMPK/NF-κB pathway in neuropathic pain mice

References for GLP1R Rabbit pAb (A8547)

Product:

GLP1R Rabbit pAb

Journal:

Clin Sci (Lond)

Application:

IF:

6.124

Species:

Homo sapiens

PMID:

33416075

Title:

Sepsis plasma-derived exosomal miR-1-3p induces endothelial cell dysfunction by targeting SERP1

References for GLP1R Rabbit pAb (A8547)

Product:

GLP1R Rabbit pAb

Journal:

Free Radic Biol Med

Application:

IF:

7.376

Species:

Rattus norvegicus

PMID:

34737144

Title:

Glucagon-like peptide-1 receptor activation maintains extracellular matrix integrity by inhibiting the activity of mitogen-activated protein kinases and activator protein-1

References for GLP1R Rabbit pAb (A8547)

Product:

GLP1R Rabbit pAb

Journal:

Journal of cardiovascular development and disease

Application:

IHC

IF:

4.41

Species:

Mus musculus

PMID:

37754815

Title:

Liraglutide Attenuates Aortic Valve Calcification in a High-Cholesterol-Diet-Induced Experimental Calcific Aortic Valve Disease Model in Apolipoprotein E-Deficient Mice - PMC

References for GLP1R Rabbit pAb (A8547)

Product:

GLP1R Rabbit pAb

Journal:

AMERICAN JOURNAL OF TRANSLATIONAL RESEARCH

Application:

IF:

2.2

Species:

Mus musculus

PMID:

29423016