제품 > 항체 > 다중클론항체(pAb)

IRF3 Rabbit pAb (A2172)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from C6 cells using IRF3 Rabbit pAb(A2172) at 1:1000 dilution.
Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 90s.

ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A2172)

Immunohistochemistry analysis of IRF3 in paraffin-embedded mouse testis tissue using IRF3 Rabbit pAb (A2172) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

Immunohistochemistry analysis of IRF3 in paraffin-embedded rat heart tissue using IRF3 Rabbit pAb (A2172) at a dilution of 1:200 (40x lens). High pressure antigen retrieval was performed with 0.01 M citrate buffer (pH 6.0) prior to IHC staining.

ABclonal:Immunofluorescence - IRF3 Rabbit pAb (A2172)

Immunofluorescence analysis of HeLa cells using IRF3 Rabbit pAb (A2172) at dilution of 1:100. Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) (AS007) at 1:500 dilution. Blue: DAPI for nuclear staining.

Confocal immunofluorescence analysis of U-2 OS cells using IRF3 Rabbit pAb (A2172) at dilution of 1:100. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - IRF3 Rabbit pAb (A2172)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg IRF3 antibody (A2172). Western blot was performed from the immunoprecipitate using IRF3 antibody (A2172) at a dilution of 1:1000.

Review

Overview

Product name	IRF3 Rabbit pAb
Catalog No.	A2172
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human IRF3 (NP_001184052.1).
Sequence	TQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGEEWEFEVTAFYRGRQVFQQTISCPEGLRLVGSEVGD
Gene ID
Swiss Prot
Synonyms	IIAE7; IRF3
Calculated MW	47kDa
Observed MW	45-55kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:500 - 1:1000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	C6
Cellular location	Cytoplasm, Nucleus
Customer validation	(B6) WB(Sus scrofa, Mus musculus, Homo sapiens, Mus musculus, Danio rerio) IF(Homo sapiens, Mus musculus) IP(Mus musculus)

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Research Area

IRF3 Rabbit pAb images

Western blot - IRF3 Rabbit pAb (A2172)

Immunohistochemistry - IRF3 Rabbit pAb (A2172)

Immunofluorescence - IRF3 Rabbit pAb (A2172)

Confocal immunofluorescence analysis of U-2 OS cells using IRF3 Rabbit pAb (A2172) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunoprecipitation - IRF3 Rabbit pAb (A2172)

* For research use only. Not for therapeutic or diagnostic purposes.

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Reviews List

Product Protocols

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Molecular Immunology

Application:

IF:

4.407

Species:

Sus scrofa

PMID:

31351410

Title:

Porcine deltacoronavirus nsp15 antagonizes interferon-β production independently of its endoribonuclease activity.

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Nano Res

Application:

IF:

8.897

Species:

Mus musculus

PMID:

33391623

Title:

Manganese nanodepot augments host immune response against coronavirus

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Sci Signal

Application:

IF:

8.19

Species:

Mus musculus

PMID:

33850055

Title:

DDAH2 suppresses RLR-MAVS-mediated innate antiviral immunity by stimulating nitric oxide-activated, Drp1-induced mitochondrial fission

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Front Immunol

Application:

IF:

7.56

Species:

Homo sapiens

PMID:

32636833

Title:

HTNV Sensitizes Host Toward TRAIL-Mediated Apoptosis-A Pivotal Anti-hantaviral Role of TRAIL

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Redox biology

Application:

IF:

10.78

Species:

Mus musculus

PMID:

35724543

Title:

STING signaling sensing of DRP1-dependent mtDNA release in kupffer cells contributes to lipopolysaccharide-induced liver injury in mice - PMC

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Autophagy

Application:

IF:

13.39

Species:

Homo sapiens

PMID:

36779599

Title:

Porcine reproductive and respiratory syndrome virus degrades DDX10 via SQSTM1/p62-dependent selective autophagy to antagonize its antiviral activity

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Journal of neuroinflammation

Application:

IF:

9.58

Species:

Homo sapiens

PMID:

37752509

Title:

Type I/type III IFN and related factors regulate JEV infection and BBB endothelial integrity - PMC

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

PLoS pathogens

Application:

IF:

7.46

Species:

Homo sapiens

PMID:

37956198

Title:

SARS-CoV-2 Nsp8 suppresses MDA5 antiviral immune responses by impairing TRIM4-mediated K63-linked polyubiquitination - PMC

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Inflammation

Application:

IF:

4.65

Species:

Homo sapiens

PMID:

37432596

Title:

KPNA2 Silencing, Regulated by E3 Ubiquitin Ligase FBXW7, Alleviates Endothelial Dysfunction and Inflammation Through Inhibiting the Nuclear Translocation of p65

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Nature

Application:

IF:

64.8

Species:

Homo sapiens

PMID:

36265513

Title:

Recognition of cyclic dinucleotides and folates by human SLC19A1

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Journal of immunology

Application:

IF:

5.426

Species:

Homo sapiens, Mus musculus

PMID:

30952814

Title:

The Otubain YOD1 Suppresses Aggregation and Activation of the Signaling Adaptor MAVS through Lys63-Linked Deubiquitination.

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Journal of Virology

Application:

WB, IF

IF:

6.549

Species:

Homo sapiens

PMID:

28566380

Title:

Seneca Valley Virus Suppresses Host Type I Interferon Production by Targeting Adaptor Proteins MAVS, TRIF, and TANK for Cleavage

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Advanced Science

Application:

IF:

15.1

Species:

Homo sapiens

PMID:

38009491

Title:

Cytoplasmic Escape of Mitochondrial DNA Mediated by Mfn2 Downregulation Promotes Microglial Activation via cGasSting Axis in Spinal Cord Injury

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Antiviral Research

Application:

IF:

10.103

Species:

Mus musculus

PMID:

32360948

Title:

Artemisinin inhibits the replication of flaviviruses by promoting the type I interferon production

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Cellular and Molecular Immunology

Application:

WB, IF

IF:

22.096

Species:

Mus musculus

PMID:

27593482

Title:

Neddylation is required for herpes simplex virus type I (HSV-1)-induced early phase interferon-beta production.

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

Journal of virology

Application:

IF:

6.54

Species:

Homo sapiens

PMID:

35980206

Title:

UBR5 Acts as an Antiviral Host Factor against MERS-CoV via Promoting Ubiquitination and Degradation of ORF4b

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

The Journal of biological chemistry

Application:

WB, IP

IF:

5.486

Species:

Mus musculus

PMID:

35490780

Title:

ERK/RSK-mediated phosphorylation of Y-box binding protein-1 aggravates diabetic cardiomyopathy by suppressing its interaction with deubiquitinase OTUB1

References for IRF3 Rabbit pAb (A2172)

Product:

IRF3 Rabbit pAb

Journal:

The American journal of Chinese medicine

Application:

IF:

5.7

Species:

Homo sapiens

PMID:

38480499