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IRF3 Rabbit pAb (A11118)

Tested applications:WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP InhibitionReactivity:Human, Mouse, Rat

Western blot analysis of lysates from 293T cells using IRF3 Rabbit pAb (A11118) at 1:1000 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) (AS014) at 1:10000 dilution.
Lysates/proteins: 25 μg per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit (RM00020).
Exposure time: 1s.

ABclonal:Immunohistochemistry - IRF3 Rabbit pAb (A11118)

Immunohistochemistry analysis of paraffin-embedded Human lung cancer using IRF3 Rabbit pAb (A11118) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

Immunohistochemistry analysis of paraffin-embedded Human esophageal cancer using IRF3 Rabbit pAb (A11118) at dilution of 1:100 (40x lens). Microwave antigen retrieval performed with 0.01M PBS Buffer (pH 7.2) prior to IHC staining.

ABclonal:Immunofluorescence - IRF3 Rabbit pAb (A11118)

Immunofluorescence analysis of HeLa cells using IRF3 Rabbit pAb (A11118) at dilution of 1:100 (40x lens). Secondary antibody: ABflo® 488-conjugated Goat Anti-Rabbit IgG (H+L) (AS073) at 1:500 dilution. Blue: DAPI for nuclear staining.

ABclonal:Immunoprecipitation - IRF3 Rabbit pAb (A11118)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg IRF3 antibody (A11118). Western blot was performed from the immunoprecipitate using IRF3 (A11118) at a dilution of 1:1000.

ABclonal:Chromatin Immunoprecipitation - IRF3 Rabbit pAb (A11118)

Chromatin immunoprecipitation analysis of extracts of HCT116 cell line, using IRF3 rabbit polyclonal antibody (A11118) and rabbit IgG. The amount of immunoprecipitated DNA was checked by quantitative PCR. Histogram was constructed by the ratios of the immunoprecipitated DNA to the input.

Overview

Product name	IRF3 Rabbit pAb
Catalog No.	A11118
Host species	Rabbit
Purification method	Affinity purification
Isotype	IgG

Background

This gene encodes a member of the interferon regulatory transcription factor (IRF) family. The encoded protein is found in an inactive cytoplasmic form that upon serine/threonine phosphorylation forms a complex with CREBBP. This complex translocates to the nucleus and activates the transcription of interferons alpha and beta, as well as other interferon-induced genes. The protein plays an important role in the innate immune response against DNA and RNA viruses. Mutations in this gene are associated with Encephalopathy, acute, infection-induced, herpes-specific, 7.

Immunogen information

Immunogen	Recombinant fusion protein containing a sequence corresponding to amino acids 100-200 of human IRF3 (NP_001184051.1).
Sequence	PHDPHKIYEFVNSGVGDFSQPDTSPDTNGGGSTSDTQEDILDELLGNMVLAPLPDPGPPSLAVAPEPCPQPLRSPSLDNPTPFPNLGPSENPLKRLLVPGE
Gene ID
Swiss Prot
Synonyms	IIAE7; IRF3
Calculated MW	47kDa
Observed MW	47kDa

Applications

Reactivity	Human, Mouse, Rat
Tested applications	WB IHC-P IF/ICC IP ChIP ChIP-seq RIP FC FC(Intra)ELISA MeDIP Nucleotide Array DB FACS CoIP CUT&Tag meRIP Inhibition
Recommended dilution	WB 1:1000 - 1:5000 IHC-P 1:50 - 1:200 IF/ICC 1:50 - 1:200 IP 0.5μg-4μg antibody for 200μg-400μg extracts of whole cells ELISA Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements. ChIP 5μg antibody for 10μg-15μg of Chromatin
Storage buffer	Store at -20℃. Avoid freeze / thaw cycles. Buffer: PBS with 0.09% Sodium azide, 50% glycerol, pH7.3.
Key application	Western blotting Immunohistochemistry Immunofluorescence Immunoprecipitation
Positive samples	293T
Cellular location	Cytoplasm, Nucleus.
Customer validation	WB(Homo sapiens, Mus musculus, Mesocricetus auratus, Sus scrofa, Mus musculus, Rattus norvegicus) IHC(Homo sapiens) IF(Mus musculus)

Documents

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Research Area

IRF3 Rabbit pAb images

Western blot - IRF3 Rabbit pAb (A11118)

Immunohistochemistry - IRF3 Rabbit pAb (A11118)

Immunofluorescence - IRF3 Rabbit pAb (A11118)

Immunoprecipitation - IRF3 Rabbit pAb (A11118)

Immunoprecipitation analysis of 300 μg extracts of 293T cells using 3 μg IRF3 antibody (A11118). Western blot was performed from the immunoprecipitate using IRF3 (A11118) at a dilution of 1:1000.

Chromatin Immunoprecipitation - IRF3 Rabbit pAb (A11118)

* For research use only. Not for therapeutic or diagnostic purposes.

Product Protocols

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Virologica Sinica

Application:

IF:

6.94

Species:

Homo sapiens

PMID:

36182074

Title:

Zika virus non-structural protein 4B interacts with DHCR7 to facilitate viral infection

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Cell death & disease

Application:

IF:

9.68

Species:

Homo sapiens

PMID:

36002459

Title:

Apoptotic caspase inhibits innate immune signaling by cleaving NF-κBs in both Mammals and Flies - PMC

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Journal of virology

Application:

IF:

6.54

Species:

Mus musculus,Homo sapiens,Mesocricetus auratus

PMID:

36326274

Title:

Substitution of S179P in the Lyssavirus Phosphoprotein Impairs Its Interferon Antagonistic Function

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Journal of virology

Application:

IF:

6.54

Species:

Sus scrofa

PMID:

36448799

Title:

Different Mechanisms Are Utilized by Coronavirus Transmissible Gastroenteritis Virus To Regulate Interferon Lambda 1 and Interferon Lambda 3 Production

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Virology journal

Application:

IF:

5.91

Species:

Homo sapiens

PMID:

37005687

Title:

ARIH1 inhibits influenza A virus replication and facilitates RIG-I dependent immune signaling by interacting with SQSTM1/p62 - PMC

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Aging cell

Application:

IF:

Species:

Mus musculus

PMID:

36949552

Title:

The reverse transcriptase inhibitor 3TC protects against age‐related cognitive dysfunction

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Frontiers in oncology

Application:

IHC

IF:

5.73

Species:

Homo sapiens

PMID:

37182129

Title:

Construction of an interferon regulatory factors-related risk model for predicting prognosis, immune microenvironment and immunotherapy in clear cell renal cell carcinoma - PMC

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Plos One

Application:

IF:

3.752

Species:

Homo sapiens

PMID:

24260525

Title:

Ubiquitin-specific proteases 25 negatively regulates virus-induced type I interferon signaling

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Nature communications

Application:

IF:

17.69

Species:

Homo sapiens, Mus musculus

PMID:

37429863

Title:

ARIH1 activates STING-mediated T-cell activation and sensitizes tumors to immune checkpoint blockade - PMC

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Small (Weinheim an der Bergstrasse, Germany)

Application:

IF:

15.15

Species:

Homo sapiens

PMID:

38009480

Title:

MnAntiCTLA4CREKASericin Nanotheragnostics for Enhanced Magnetic Resonance Imaging and Tumor Immunotherapy

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

Neural plasticity

Application:

IF:

3.14

Species:

Rattus norvegicus

PMID:

36910013

Title:

Electroacupuncture Zusanli (ST36) Relieves Somatic Pain in Colitis Rats by Inhibiting Dorsal Root Ganglion Sympathetic-Sensory Coupling and Neurogenic Inflammation - PMC

References for IRF3 Rabbit pAb (A11118)

Product:

IRF3 Rabbit pAb

Journal:

iScience

Application:

IF:

6.1

Species:

Mus musculus

PMID:

35712074