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ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS037)

Publications (31) Datasheet

Tested applications:WBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibitionReactivity:

ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS037)

Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by a further incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS037, dilution 1:800) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.

ABclonal:Flow CytoMetry - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS037)

Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Hep G2 cells (right) were surface-stained with Mouse Anti-Human GPC3 mAb (4 μg/mL, orange line) or secondary antibody only (blue line). Non-fluorescently stained HepG2 and K-562 cells were used as blank control (red line). ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS037, 1:500)was used as a secondary antibody.

Overview

Product nameABflo® 488-conjugated Goat anti-Mouse IgG (H+L)
Catalog No.AS037
Host speciesGoat
Purification methodAffinity purification
IsotypeIgG
Secondary antibodies are affinity-purified antibodies which will work with target-specific primary antibody in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies . Most commonly, secondary antibodies are generated by immunizing the host animal (different from host species of primary antibody) with a pooled population of normal immunoglobulins from the host species of primary antibody and can be further purified and modified (i.e. antibody fragmentation, label conjugation, etc.) to ensure well-characterized specificity to corresponding normal immunoglobulins.
ImmunogenMouse IgG
SequenceEmail for sequence
Gene ID
Swiss Prot
Synonyms
Calculated MW
Observed MW
Reactivity
Tested applicationsWBIHC-PIF/ICCIPChIPChIP-seqRIPFCFC(Intra)ELISAMeDIPNucleotide ArrayDBFACSCoIPCUT&TagmeRIPInhibition
Recommended dilution
  • IF/ICC 1:50 - 1:200
  • FC 1:100 - 1:500
Storage bufferStore at -20℃. Avoid freeze / thaw cycles.
Buffer: PBS with 0.02% sodium azide, 1% BSA, 50% glycerol, pH7.3.
Key applicationImmunofluorescence    Flow Cytometry    
Positive samples
Cellular location
Customer validation

IF(Rattus norvegicus, Zea mays, Homo sapiens, Mus musculus, Crassostrea gigas, Ostreidae, Mus musculus, Oryctolagus cuniculus)

IHC(Homo sapiens, Crassostrea gigas)

WB(Homo sapiens, Saccharomyces cerevisiae, Mus musculus)

FC(Crassostrea gigas)

Other(Mus musculus )

FC(Homo sapiens)

Enzyme assay(Danio rerio)

Documents

Certificate of Compliance

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    ABclonal:Immunofluorescence - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS037)}

    Immunofluorescence - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS037)

    Confocal imaging of HeLa cells using α-Tubulin Mouse mAb (AC012, dilution 1:400) followed by a further incubation with ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS037, dilution 1:800) (Green). DAPI was used for nuclear staining (Blue). Objective: 100x.
    ABclonal:Flow CytoMetry - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS037)}

    Flow CytoMetry - ABflo® 488-conjugated Goat anti-Mouse IgG (H+L) (AS037)

    Flow cytometry: 1X10^6 K-562 cells (negative control, left) and Hep G2 cells (right) were surface-stained with Mouse Anti-Human GPC3 mAb (4 μg/mL, orange line) or secondary antibody only (blue line). Non-fluorescently stained HepG2 and K-562 cells were used as blank control (red line). ABflo® 488-conjugated Goat Anti-Mouse IgG (H+L) (AS037, 1:500)was used as a secondary antibody.

    * For research use only. Not for therapeutic or diagnostic purposes.

    Publishing research using AS037? Please let us know so that we can cite the reference in this datasheet.